Hereditary tyrosinemia type 1 metabolites impair DNA excision repair pathways
| Autor: | Piet J. Pretorius, Gerhard Koekemoer, E. van Dyk, A. Steenkamp |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
DNA Repair
Phenylpyruvic Acids DNA repair Biophysics Biology Biochemistry Cell Line medicine Humans Molecular Biology chemistry.chemical_classification Tyrosinemias Metabolic disorder Hydrogen Peroxide Cell Biology Base excision repair medicine.disease DNA excision Molecular biology Heptanoates Comet assay Enzyme chemistry Fumarylacetoacetate hydrolase Comet Assay Nucleotide excision repair |
| Zdroj: | Biochemical and Biophysical Research Communications. 401:32-36 |
| ISSN: | 0006-291X |
| Popis: | Hereditary tyrosinemia type 1 is an autosomal recessive metabolic disorder, which is caused by a defective fumarylacetoacetate hydrolase enzyme, and consequently metabolites such as succinylacetone and p-hydroxyphenylpyruvate accumulate. We used a modified comet assay to determine the effect of these metabolites on base- and nucleotide excision repair pathways. Our results indicate that the metabolites affected the repair mechanisms differently, since the metabolites had a bigger detrimental effect on BER than on NER. |
| Databáze: | OpenAIRE |
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