Genetically modified filamentous phage as bactericidal agents: a pilot study
| Autor: | S. Hagens, U. Blasi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
biology
viruses Phagemid Bacterial Infections Genetic Therapy medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Virology Genetically modified organism Microbiology Bacteriophage Viral Proteins Restriction enzyme Lytic cycle Holin Escherichia coli medicine Humans Deoxyribonucleases Type II Site-Specific Genetic Engineering Bacteria Bacteriophage M13 |
| Zdroj: | Letters in Applied Microbiology. 37:318-323 |
| ISSN: | 0266-8254 |
| DOI: | 10.1046/j.1472-765x.2003.01400.x |
| Popis: | Aims: To evaluate the ability of a filamentous phage encoding lethal proteins to kill bacteria without host-cell lysis. Methods and Results: Bacterial survival was determined after infection of a growing Escherichia coli culture with phage M13 encoding either the restriction endonuclease BglII gene or modified phage λS holin genes. The genetically engineered phage exerted a high killing efficiency while leaving the cells structurally intact. When compared with a lytic phage, the release of endotoxin was minimized after infection with the genetically modified phages. Conclusions: Genetically engineered phage can be used for efficient killing, concomitantly minimizing endotoxin release. Significance and Impact of the Study: This feasibility study provides a possible strategy for the use of genetically engineered phage as bactericidal agents by optimizing the advantages and minimizing potential risks such as release of pyrogenic cell wall components. |
| Databáze: | OpenAIRE |
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