Further analysis of the structure and immunological activity of an RG-I type pectin from Panax ginseng
Autor: | Lin Sun, Yuying Fan, Shanshan Li, Yifa Zhou, Jingjing Zhu, Xu Zhang, Li Ji, Guihua Tai |
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Rok vydání: | 2012 |
Předmět: |
Arabinose
food.ingredient Polymers and Plastics Pectin Rhamnose Panax Lymphocyte proliferation Polysaccharide Methylation Mice chemistry.chemical_compound Hydrolysis food Phagocytosis Arabinogalactan Materials Chemistry Animals Trifluoroacetic Acid Lymphocytes Cell Proliferation chemistry.chemical_classification Periodic Acid Organic Chemistry chemistry Biochemistry Neutral Red Galactose Macrophages Peritoneal Pectins Oxidation-Reduction Spleen |
Zdroj: | Carbohydrate Polymers. 89:519-525 |
ISSN: | 0144-8617 |
Popis: | In this paper, we further analysed the structure of a type I rhamnogalacturonan (RG-I) pectin (WGPA-2-RG) fractionated from ginseng polysaccharides. Methylation and periodate oxidation analyses showed that WGPA-2-RG has a backbone consisting of alternating rhamnose (Rha) and galacturonic acid (GalA) residues and side chains consisting of type II arabinogalactan (AG-II). Partial acidic hydrolysis for 6h completely removed arabinose (Ara), partial galactose (Gal), but little GalA and Rha. During partial hydrolysis, the molecular weight of WGPA-2-RG decreased smoothly, suggesting that the Ara and cleavable Gal residues exist on the surface of the molecule, while GalA and Rha residues exist in the core of the molecule. The bioactivity assay showed that the arabinogalactan side chains of WGPA-2-RG are essential structures for stimulating NO secretion and lymphocyte proliferation. However, removal of the Ara and Gal residues through hydrolysis did not appreciably affect the ability of WGPA-2-RG to enhance macrophage phagocytosis. |
Databáze: | OpenAIRE |
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