Global transgenerational gene expression dynamics in two newly synthesized allohexaploid wheat (Triticum aestivum) lines
| Autor: | Huakun Zhang, Wei Huang, Chunming Xu, Bao Liu, Fangpu Han, Jianhua Guo, Na Zhao, Bo Zhu, Xiaofang Zhong, Jinsong Pang, Bao Qi |
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| Jazyk: | angličtina |
| Předmět: |
Physiology
Gene Expression Plant Science Genes Plant Real-Time Polymerase Chain Reaction Genome General Biochemistry Genetics and Molecular Biology Evolution Molecular Polyploidy Structural Biology Gene Expression Regulation Plant Gene expression Genetic variation Aegilops tauschii Common wheat Gene lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics In Situ Hybridization Triticum Oligonucleotide Array Sequence Analysis Genetics Regulation of gene expression biology Agricultural and Biological Sciences(all) Reverse Transcriptase Polymerase Chain Reaction Biochemistry Genetics and Molecular Biology(all) Genetic Variation food and beverages Cell Biology biology.organism_classification lcsh:Biology (General) DNA microarray General Agricultural and Biological Sciences Developmental Biology Biotechnology Research Article |
| Zdroj: | BMC Biology BMC Biology, Vol 10, Iss 1, p 3 (2012) |
| ISSN: | 1741-7007 |
| DOI: | 10.1186/1741-7007-10-3 |
| Popis: | Background Alteration in gene expression resulting from allopolyploidization is a prominent feature in plants, but its spectrum and extent are not fully known. Common wheat (Triticum aestivum) was formed via allohexaploidization about 10,000 years ago, and became the most important crop plant. To gain further insights into the genome-wide transcriptional dynamics associated with the onset of common wheat formation, we conducted microarray-based genome-wide gene expression analysis on two newly synthesized allohexaploid wheat lines with chromosomal stability and a genome constitution analogous to that of the present-day common wheat. Results Multi-color GISH (genomic in situ hybridization) was used to identify individual plants from two nascent allohexaploid wheat lines between Triticum turgidum (2n = 4x = 28; genome BBAA) and Aegilops tauschii (2n = 2x = 14; genome DD), which had a stable chromosomal constitution analogous to that of common wheat (2n = 6x = 42; genome BBAADD). Genome-wide analysis of gene expression was performed for these allohexaploid lines along with their parental plants from T. turgidum and Ae. tauschii, using the Affymetrix Gene Chip Wheat Genome-Array. Comparison with the parental plants coupled with inclusion of empirical mid-parent values (MPVs) revealed that whereas the great majority of genes showed the expected parental additivity, two major patterns of alteration in gene expression in the allohexaploid lines were identified: parental dominance expression and non-additive expression. Genes involved in each of the two altered expression patterns could be classified into three distinct groups, stochastic, heritable and persistent, based on their transgenerational heritability and inter-line conservation. Strikingly, whereas both altered patterns of gene expression showed a propensity of inheritance, identity of the involved genes was highly stochastic, consistent with the involvement of diverse Gene Ontology (GO) terms. Nonetheless, those genes showing non-additive expression exhibited a significant enrichment for vesicle-function. Conclusions Our results show that two patterns of global alteration in gene expression are conditioned by allohexaploidization in wheat, that is, parental dominance expression and non-additive expression. Both altered patterns of gene expression but not the identity of the genes involved are likely to play functional roles in stabilization and establishment of the newly formed allohexaploid plants, and hence, relevant to speciation and evolution of T. aestivum. |
| Databáze: | OpenAIRE |
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