Marine Microorganisms for Biocatalysis: Selective Hydrolysis of Nitriles with a Salt-Resistant Strain of Meyerozyma guilliermondii
| Autor: | Immacolata Serra, Luisa Pellegrino, Francesco Molinari, Concetta Compagno, Loana Musso, Claudia Capusoni |
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| Přispěvatelé: | Serra, I, Capusoni, C, Molinari, F, Musso, L, Pellegrino, L, Compagno, C |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Nitrile Biology 01 natural sciences Applied Microbiology and Biotechnology Nitrilase 03 medical and health sciences chemistry.chemical_compound Hydrolysis Biotransformation Aminohydrolases Cyclohexanes 010608 biotechnology Meyerozyma guilliermondii Nitriles Organic chemistry Seawater Bioprocess Biocatalysi Yeast Benzonitrile 030104 developmental biology chemistry Biocatalysis Saccharomycetales Marine yeast |
| Zdroj: | Marine biotechnology (New York, N.Y.). 21(2) |
| ISSN: | 1436-2236 |
| Popis: | A screening among marine yeasts was carried out for nitrile hydrolyzing activity. Meyerozyma guilliermondii LM2 (UBOCC-A-214008) was able to efficiently grow on benzonitrile and cyclohexanecarbonitrile (CECN) as sole nitrogen sources. A two-step one-pot method for obtaining cells of M. guilliermondii LM2(UBOCC-A-214008) endowed with high nitrilase activity was established; the resulting whole cells converted different nitriles with high molar conversions and showed interesting enantioselectivity toward racemic substrates. Nitrilase from M. guilliermondii LM2(UBOCC-A-214008) displayed high activity on aromatic substrates, but also arylaliphatic and aliphatic substrates were accepted. Salt-resistant M. guilliermondii LM2(UBOCC-A-214008) was used in media with different salinity, being highly active up to 1.5M NaCl concentration. Finally, hydrolysis of nitriles was efficiently performed using a bioprocess (yeast growth and biotransformation with resting cells) entirely carried out in seawater. |
| Databáze: | OpenAIRE |
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