Phorbol ester-induced shedding of intercellular adhesion molecule-1 (ICAM-1) on erythroleukemic K 562 cells
| Autor: | Siegfried Ansorge, Thomas Schlüter, Simone Fritz, Peter Schönfeld, Dirk Reinhold, Ralf Bohnensack, Frank Striggow |
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| Rok vydání: | 1996 |
| Předmět: |
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Western Cell Intercellular Adhesion Molecule-1 Gene Expression Enzyme-Linked Immunosorbent Assay Cell Line Flow cytometry chemistry.chemical_compound Tumor Cells Cultured medicine Fluorescence microscope Humans Fluorescent Antibody Technique Indirect Molecular Biology Phorbol myristate acetate ICAM-1 Dose-Response Relationship Drug medicine.diagnostic_test Tumor Necrosis Factor-alpha Chemistry Intercellular adhesion molecule-1 Adhesion Cell Biology Flow Cytometry Molecular biology Tumor necrosis factor-α Cell biology Kinetics medicine.anatomical_structure Microscopy Fluorescence Phorbol Tetradecanoylphorbol Acetate Leukemia Erythroblastic Acute Intracellular |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1312(3):255-261 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(96)00039-0 |
| Popis: | The effect of phorbol 12-myristate 13-acetate (PMA) on the expression and shedding of intercellular adhesion molecule-1 (ICAM-1) was investigated on the hematopoietic cell lines K 562 and U 937 using flow cytometry, fluorescence microscopy and ELISA technique. At low concentration of 1 nM, PMA stimulated the expression of ICAM-1 on the cell surface about 4-fold within 24 h, whereas a short-term treatment with 101) nM PMA led to the shedding of 35% of ICAM-1 from the surface of K 562 cells. The release of surface ICAM-1 was found on single cells by fluorescence microscopy to be a uniform process proceeding within 15 min. The shedding of ICAM-1 correlated with elevated levels of sICAM-1 in the supernatants of cultured cells. Also on K 562 cells stimulated by TNF-α, a PMA-induced release of ICAM-1 was observed in addition to the known spontaneous shedding. In contrast to the results with K 562 cells, no PMA-induced shedding of ICAM-1 was found on U 937 cells. This indicates a cell-specific process for K 562 cells. The PMA-mediated release of ICAM-1 from K 562 cells suggests that the shedding process does not only occur in parallel to the surface expression of ICAM-1, but may be controlled by particular mechanisms of down-regulation. |
| Databáze: | OpenAIRE |
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