Comparison of rRNA-based reverse transcription PCR and rDNA-based PCR for the detection of streptococci in root canal infections
| Autor: | Ericka Tavares Pinheiro, Vitor Cesar Nakamura, Giulio Gavini, L. C. Prado, Amanda da Costa da Silveira, Marcia Pinto Alves Mayer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Biology DNA Ribosomal law.invention 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine law Complementary DNA Root canal treatment Humans General Dentistry Polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction RNA Reproducibility of Results Streptococcus 030206 dentistry Original Articles Ribosomal RNA 16S ribosomal RNA biology.organism_classification Molecular biology Root Canal Therapy Reverse transcription polymerase chain reaction lcsh:RK1-715 genomic DNA RNA Bacterial RNA Ribosomal lcsh:Dentistry Dental Pulp Cavity Bacteria |
| Zdroj: | Journal of Applied Oral Science, Volume: 27, Article number: e20180256, Published: 29 JUL 2019 Journal of Applied Oral Science v.27 2019 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP Journal of Applied Oral Science, Vol 27 Journal of Applied Oral Science Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) |
| Popis: | Objective The rDNA-based method is unable to distinguish between alive and dead cells. Alternatively, bacterial viability can be assessed by molecular methods based on ribosomal RNA (rRNA). Therefore, this study aimed to detect viable streptococci in root canal samples using rRNA-based reverse transcription polymerase chain reaction (RT-PCR), compared to an rDNA-based PCR assay. Methodology Microbiological root canal samples were obtained from 32 teeth with primary endodontic infections before (S1) and after chemomechanical preparation (S2), and after removal of intracanal medication (S3). RNA and DNA were extracted, and complementary DNA (cDNA) was synthesized from RNA using RT reaction. cDNA and genomic DNA were subjected to PCR with primers complementary to the 16S rRNA sequences of Streptococcus spp. McNemar’s test was used to compare the detection rate of both assays (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|