Expression Profiling of Cytokine, Cholinergic Markers, and Amyloid-β Deposition in the APPSWE/PS1dE9 Mouse Model of Alzheimer’s Disease Pathology
| Autor: | Nieves Salvador, Marcella Reale, Marta Di Nicola, Nagnedra Sastry Yarla, Erica Costantini, Chiara D'Angelo, George Perry, Mohammad Amjad Kamal |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetically modified mouse medicine.medical_specialty Aging medicine.medical_treatment Gene Expression Mice Transgenic Biology cholinergic markers Receptors Nicotinic pro-inflammatory cytokines Real-Time Polymerase Chain Reaction Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Internal medicine medicine Animals Entorhinal Cortex Humans Amyloid beta-Peptides General Neuroscience Gene Expression Profiling Interleukin APPswe transgenic mice General Medicine Entorhinal cortex Immunohistochemistry Olfactory Bulb Olfactory bulb Psychiatry and Mental health Clinical Psychology Aβ accumulation Disease Models Animal 030104 developmental biology Cytokine Endocrinology Butyrylcholinesterase Acetylcholinesterase Cholinergic Cytokines Tumor necrosis factor alpha Geriatrics and Gerontology Alzheimer’s disease 030217 neurology & neurosurgery Research Article |
| Zdroj: | Journal of Alzheimer's Disease |
| ISSN: | 1875-8908 1387-2877 |
| Popis: | Background: Alzheimer’s disease (AD), a neurodegenerative disease, is associated with dysfunction of the olfactory and the entorhinal cortex of the brain that control memory and cognitive functions and other daily activities. Pro-inflammatory cytokines, amyloid-β (Aβ), and the cholinergic system play vital roles in the pathophysiology of AD. However, the role of changes in cholinergic system components, Aβ accumulation, and cytokines in both the olfactory and entorhinal cortex is not known clearly. Objective: The present study is aimed to evaluate the changes of cholinergic system components, Aβ accumulation, and cytokines in both the olfactory bulb (OB) and entorhinal cortex (EC) of young and aged APPSWE/PS1dE9 transgenic (Tg) mice. Methods: We have explored the changes of cholinergic system components, Aβ accumulation, and expression profiling of cytokines in the OB and EC of aged APPswe transgenic mice and age-matched wild type mice using quantitative Real-Time PCR assays and immunohistochemistry techniques. Results: In aged Tg mice, a significant increase of expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and chemokine MCP1 (p |
| Databáze: | OpenAIRE |
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