Dysbacteriosis-induced LPS elevation disturbs the development of muscle progenitor cells by interfering with retinoic acid signaling
| Autor: | Denglu Long, Xuesong Yang, Chaojie Wang, Jinhuan Song, Ziguang Li, Lingsen You, Beate Brand-Saberi, Guang Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male Retinoic acid Tretinoin Chick Embryo Gut flora Muscle Development digestive system Biochemistry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Somitogenesis Genetics medicine Limb development Animals Progenitor cell Molecular Biology Cell Proliferation chemistry.chemical_classification Reactive oxygen species biology Myogenesis Muscles Stem Cells Cell Differentiation biology.organism_classification medicine.disease Cell biology Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology chemistry Dysbiosis Female 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 34(5) |
| ISSN: | 1530-6860 |
| Popis: | Whether myogenesis is affected by the maternal gut dysbacteriosis still remains ambiguous. In this study, first we show the elevated level of lipopolysaccharides (LPS) in a gut microbiota dysbiosis mouse model. Second, we demonstrate that the diameter of muscle fibers, limb development, and somitogenesis were inhibited in both the gut microbiota dysbiosis and LPS exposed mice and chicken embryos. These might be due to LPS disturbed the cell survival and key genes which regulate the somitogenesis and myogenesis. RNA sequencing and subsequent validation experiments verified that retinoic acid (RA) signaling perturbation was mainly responsible for the aberrant somite formation and differentiation. Subsequently, we found that LPS-induced reactive oxygen species (ROS generation and antioxidant genes such as Nrf2, AKR1B10) contributed to the above -mentioned interference with RA signaling. These findings highlight that the gut microbiota homeostasis is also involved in regulating the development of muscle progenitor cells during pregnancy. |
| Databáze: | OpenAIRE |
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