Telomerase activity in surgical specimens and fine-needle aspiration biopsies from hyperplastic and neoplastic human thyroid tissues
Autor: | Asa K. Velin, Anders Herder, Lena M. Trulsson, Peter Söderkvist, Staffan Smeds, Anders Rüter |
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Rok vydání: | 2003 |
Předmět: |
Adult
Male Telomerase Pathology medicine.medical_specialty Enzyme-Linked Immunosorbent Assay Malignancy Polymerase Chain Reaction Diagnosis Differential Adenocarcinoma Follicular Biopsy Biomarkers Tumor medicine Humans Thyroid Neoplasms Thyroid cancer Aged Aged 80 and over medicine.diagnostic_test business.industry Biopsy Needle Thyroid Cancer General Medicine Middle Aged Hyperplasia medicine.disease Carcinoma Papillary medicine.anatomical_structure Fine-needle aspiration Female Surgery business |
Zdroj: | The American Journal of Surgery. 186:83-88 |
ISSN: | 0002-9610 |
Popis: | Background: Telomerase activity (TA) indicates malignancy, but activated lymphocytes also express TA. Correlation between TA in thyroid tissues and fine-needle aspiration (FNA) samples and knowledge about TA in adjacent tissue are of importance. Methods: The telomeric repeat amplification protocol assay followed by enzyme-linked immunosorbent assay detection was performed on 78 thyroid cases including 53 suspected malignancies, preoperative and perioperative FNA specimens, and adjacent tissue. Results: Benign lesions in cancer-suspected cases were TA negative. Eight of 13 papillary (62%) and 4 of 5 follicular (80%) tumors were TA positive (TA+). Lower TA was observed in conventional papillary cancer than in follicular, tall cell variant of papillary and anaplastic cancers. Adjacent tissues with lymphocyte infiltration were TA+ in 9 of 17 cases (53%). Nine of 65 adjacent tissues (14%) were TA+. Three of 6 preoperative and 9 of 11 perioperative FNA samples from malignant tumors corresponded to the tissue TA. Conclusions: High TA may reflect more severe thyroid cancer. Telomerase activity in FNA biopsies does not add reliable diagnostic information, and presence of lymphocytes can give false-positive results. |
Databáze: | OpenAIRE |
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