Antifungal effect of volatile organic compounds produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 on oxidative stress and mitochondrial dysfunction of Ceratocystis fimbriata
| Autor: | Li Zongyun, Jianheng Guo, Chun-Mei Zhang, Sheng Qin, Ming-Jie Xu, Yu Zhang, Ke Xing, Zhaozhong Feng, Xue Peng, Tengjie Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antifungal Agents Hypha Health Toxicology and Mutagenesis education medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Ascomycota Pseudomonas medicine Extracellular Plant Diseases chemistry.chemical_classification Volatile Organic Compounds Reactive oxygen species Ergosterol biology Chemistry fungi General Medicine Pseudomonas chlororaphis biology.organism_classification Ceratocystis Mitochondria Oxidative Stress 010602 entomology 030104 developmental biology Ceratocystis fimbriata Agronomy and Crop Science Oxidative stress Intracellular |
| Zdroj: | Pesticide Biochemistry and Physiology. 173:104777 |
| ISSN: | 0048-3575 |
| DOI: | 10.1016/j.pestbp.2021.104777 |
| Popis: | Ceratocystis fimbriata is the pathogen of black rot disease, which widely exists in sweet potato producing areas all over the world. The antifungal activity of volatile organic compounds (VOCs) released by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 against C. fimbriata was reported in our previous study. In this study, we attempted to reveal the underlying antifungal mechanism of SPS-41 volatiles. Our results showed that the VOCs released by SPS-41 caused the morphological change of hyphae, destroyed the integrity of cell membrane, reduced the content of ergosterol, and induced massive accumulation of reactive oxygen species in C. fimbriata cells. Furthermore, SPS-41 fumigation decreased the mitochondrial membrane potential, acetyl-CoA and pyruvate content of C. fimbriata cells, as well as the mitochondrial dehydrogenases activity. In addition, the VOCs generated by SPS-41 reduced the intracellular ATP content and increased the extracellular ATP content of C. fimbriata. In summary, SPS-41 fumigation exerted its antifungal activity by inducing oxidative stress and mitochondrial dysfunction in C. fimbriata. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect