Eight-Year Survey of Human Rotavirus Strains Demonstrates Circulation of Unusual G and P Types in Hungary
| Autor: | M. Új, Jon R. Gentsch, Krisztián Bányai, Ilona Mihály, György Szücs, Roger I. Glass |
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| Rok vydání: | 2004 |
| Předmět: |
Rotavirus
Microbiology (medical) Serotype Hungary Adolescent Reverse Transcriptase Polymerase Chain Reaction Infant Newborn Rotavirus Vaccines Infant Reoviridae Biology medicine.disease_cause biology.organism_classification Virology Feces Child Preschool Human rotavirus medicine Humans Child Mixed infection |
| Zdroj: | Journal of Clinical Microbiology. 42:393-397 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Between 1992 and 2000, a total of 4,173 rotavirus-positive samples were collected from two areas of Hungary. Of these, 2,020 specimens (48.4%) were analyzed for G serotype, using monoclonal antibody-based immunoassay and reverse transcription-PCR. By the two methods, 1,789 samples were specified as G1 (62%), G2 (12.2%), G3 (1.4%), G4 (6.4%), G6 (1.0%), G9 (2.9%), or mixed infection (2.6%), and the remaining 231 (11.4%) could not be G typed. The linkage between G and P type, subgroup specificity, and RNA profile was investigated with a sample subset. Among these specimens, we identified both the four globally common strains (P[8],G1 subgroup II (sgII); P[4],G2 sgI; P[8],G3 sgII; and P[8],G4 sgII) and six uncommon strains (P[6],G4 sgII; P[9],G3 sgI; P[9],G6 sgI; P[14],G6 sgI; P[8],G9 sgII; and P[8],G9 sgI). All strains with P[8], P[6], P[9], and P[14] specificities had a long electropherotype, whereas most of those carrying a P[4] specificity were associated with a short electropherotype. Although once considered to be rare, P[9],G6 and P[8],G9 rotavirus strains represent potentially important new serotypes in Hungary. |
| Databáze: | OpenAIRE |
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