Hemophagocytic Macrophages in Murine Typhoid Fever Have an Anti-Inflammatory Phenotype
| Autor: | Melissa W. McCoy, Corrella S. Detweiler, Sarah M. Moreland |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Salmonella typhimurium
Time Factors Immunology Population Yersinia pseudotuberculosis Infections Apoptosis Spleen Inflammation Biology Microbiology Immunophenotyping Mice Phagocytosis medicine Animals Yersinia pseudotuberculosis Typhoid Fever education Histiocyte Host Response and Inflammation education.field_of_study Macrophages Flow Cytometry biology.organism_classification Disease Models Animal Infectious Diseases medicine.anatomical_structure Parasitology medicine.symptom Hemophagocytosis |
| Zdroj: | Infection and Immunity. 80:3642-3649 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Histiocytes are white blood cells of the monocytic lineage and include macrophages and dendritic cells. In patients with a variety of infectious and noninfectious inflammatory disorders, histiocytes can engulf nonapoptotic leukocytes and nonsenescent erythrocytes and thus become hemophagocytes. We report here the identification and characterization of splenic hemophagocytes in a natural model of murine typhoid fever. The development of a flow-cytometric method allowed us to identify hemophagocytes based on their greater than 6N (termed 6N+) DNA content. Characterization of the 6N+ population from infected mice showed that these cells consist primarily of macrophages rather than dendritic cells and contain T lymphocytes, consistent with hemophagocytosis. Most 6N+ macrophages from Salmonella enterica serovar Typhimurium-infected mice contain intact DNA, consistent with hemophagocytosis. In contrast, most 6N+ macrophages from control mice or mice infected with a different bacterial pathogen, Yersinia pseudotuberculosis , contain damaged DNA. Finally, 6N+ splenic macrophages from S . Typhimurium-infected mice express markers consistent with an anti-inflammatory M2 activation state rather than a classical M1 activation state. We conclude that macrophages are the predominant splenic hemophagocyte in this disease model but not in Y. pseudotuberculosis -infected mice. The anti-inflammatory phenotype of hemophagocytic macrophages suggests that these cells contribute to the shift from acute to chronic infection. |
| Databáze: | OpenAIRE |
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