Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome
| Autor: | Hong-jie Liu, Hai-lan Tang, Mei-xia Fang, Li-guo Chen, Ming-ming Wang, Hua-qiang Wang |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Gene prediction Gene Expression Blood stasis Biology Umbilical vein Young Adult microRNA Gene expression Human Umbilical Vein Endothelial Cells Humans Pharmacology (medical) RNA Messenger Differential expression Cells Cultured Messenger RNA General Medicine Middle Aged Molecular biology MicroRNAs Complementary and alternative medicine Immunology Hypertension Target mrna Female sense organs |
| Zdroj: | Chinese journal of integrative medicine. 21(11) |
| ISSN: | 1672-0415 |
| Popis: | Objective Objective: To screen out blood-stasis syndrome (BSS)-associated microRNA and therefore : To screen out blood-stasis syndrome (BSS)-associated microRNA and therefore determine the possible target for treating hypertension. determine the possible target for treating hypertension. Methods Methods: A high-energy sequencing method and digital : A high-energy sequencing method and digital gene expression sequencing theory were adopted to sequence microRNA (miRNA) and messenger RNA gene expression sequencing theory were adopted to sequence microRNA (miRNA) and messenger RNA (mRNA), and to determine differential expression in human umbilical vein endothelial cells incubated with serum (mRNA), and to determine differential expression in human umbilical vein endothelial cells incubated with serum samples from hypertension patients with or without BSS, and healthy controls. The results were confi rmed using samples from hypertension patients with or without BSS, and healthy controls. The results were confi rmed using gene prediction software. gene prediction software. Results Results: A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the : A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the BSS/normal groups and BSS/non-BSS groups, respectively. Four pairs of target mRNA/miRNA were identifi ed: BSS/normal groups and BSS/non-BSS groups, respectively. Four pairs of target mRNA/miRNA were identifi ed |
| Databáze: | OpenAIRE |
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