Trapping of 2,7-Dichlorodibenzo- p -Dioxin in Aqueous Solution by Enzymatic Reaction of Fungal Manganese Peroxidase in the Presence of Polyunsaturated Fatty Acids
| Autor: | Koichi Harazono, Yoshio Watanabe, Takema Fukatsu, Ryuichiro Kurane |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipoxygenase
Chemical Fractionation Dioxins Phanerochaete Applied Microbiology and Biotechnology Microbiology Fungal Proteins Lipid peroxidation chemistry.chemical_compound Manganese peroxidase Hexanes Organic chemistry chemistry.chemical_classification Fungal protein Chromatography biology Fatty acid General Medicine Hydrogen-Ion Concentration Thiobarbiturates biology.organism_classification Peroxidases chemistry Fatty Acids Unsaturated biology.protein Lipid Peroxidation Peroxidase Polyunsaturated fatty acid |
| Zdroj: | Current Microbiology. 47:250-254 |
| ISSN: | 1432-0991 0343-8651 |
| Popis: | In the presence of polyunsaturated fatty acids, including cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), 2,7-dichlorodibenzo-p-dioxin (DCDD) was treated with manganese peroxidase (MnP) from white rot basidiomycete Phanerochaete sordida YK-624. After incubation with MnP, DCDD could not be extracted from the reaction mixture with n-hexane and was trapped in the water layer. DCDD was released by alkalification of the water layer. DCDD was also trapped after treatment with lipoxidase, which produces hydroperoxides from unsaturated lipids. The amounts of thiobarbituric acid-reactive substances produced in the MnP reactions with three highly unsaturated fatty acids were higher than the amounts produced with three fatty acids with a lower degree of unsaturation. These results suggest that a DCDD-trapping compound may be produced by peroxidation of the polyunsaturated fatty acids. |
| Databáze: | OpenAIRE |
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