Overlapping Mechanisms Promote Postsynaptic RAD-51 Filament Disassembly during Meiotic Double-Strand Break Repair
| Autor: | Federica Marini, Jordan D. Ward, Julie S. Martin, D.M. Muzzini, G. Cassata, Mark I.R. Petalcorin, Enrique Martinez-Perez, Paolo Plevani, Simon J. Boulton |
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| Rok vydání: | 2010 |
| Předmět: |
DNA Repair
DNA Single-Stranded medicine.disease_cause Protein filament chemistry.chemical_compound Meiosis medicine Animals DNA Breaks Double-Stranded Strand invasion Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Recombination Genetic Genetics Mutation biology fungi DNA Helicases Helicase Cell Biology DNA Helminth Double Strand Break Repair Cell biology DNA-Binding Proteins chemistry biology.protein Rad51 Recombinase Homologous recombination DNA |
| Zdroj: | Molecular Cell. 37:259-272 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2009.12.026 |
| Popis: | Homologous recombination (HR) is essential for repair of meiotic DNA double-strand breaks (DSBs). Although the mechanisms of RAD-51-DNA filament assembly and strand exchange are well characterized, the subsequent steps of HR are less well defined. Here, we describe a synthetic lethal interaction between the C. elegans helicase helq-1 and RAD-51 paralog rfs-1, which results in a block to meiotic DSB repair after strand invasion. Whereas RAD-51-ssDNA filaments assemble at meiotic DSBs with normal kinetics in helq-1, rfs-1 double mutants, persistence of RAD-51 foci and genetic interactions with rtel-1 suggest a failure to disassemble RAD-51 from strand invasion intermediates. Indeed, purified HELQ-1 and RFS-1 independently bind to and promote the disassembly of RAD-51 from double-stranded, but not single-stranded, DNA filaments via distinct mechanisms in vitro. These results indicate that two compensating activities are required to promote postsynaptic RAD-51 filament disassembly, which are collectively essential for completion of meiotic DSB repair. |
| Databáze: | OpenAIRE |
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