Isolation and characterization ofFrancisella novicidamutants defective in lipopolysaccharide biosynthesis
| Autor: | Catherine J Gray, Francis E. Nano, Siobhán C. Cowley |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Lipopolysaccharides
Transposable element Guinea Pigs Mutant Mutagenesis (molecular biology technique) medicine.disease_cause Microbiology Mice chemistry.chemical_compound Biosynthesis Genetics medicine Animals Francisella novicida Francisella Molecular Biology Gene Mutation biology Macrophages O Antigens biology.organism_classification Blotting Southern Mutagenesis Insertional Phenotype chemistry DNA Transposable Elements Deoxycholic Acid |
| Zdroj: | FEMS Microbiology Letters. 182:63-67 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2000.tb08874.x |
| Popis: | In order to identify genes involved in LPS biosynthesis we isolated random mutants generated by transposon insertion in Francisella novicida. The resulting mutant bank yielded mutants with three distinct LPS phenotypes, and three representative mutants were chosen for further study. One mutant that had short O-antigen chains was sensitive to serum; this mutant and one other were more sensitive to killing by deoxycholate than control strains. The third mutant was resistant to deoxycholate killing but slightly sensitive to serum. The three mutants varied in their ability to grow in macrophages. The DNA sequences interrupted by the transposon in two of the three mutants showed similarity to known LPS biosynthetic genes at the deduced amino acid level. |
| Databáze: | OpenAIRE |
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