A comparison of Chikungunya virus infection, progression, and cytokine profiles in human PMA-differentiated U937 and murine RAW264.7 monocyte derived macrophages

Autor: Bradford K. Berges, Taalin R. Hoj, E. Shannon Tass, Richard A. Robison, Israel Guerrero-Arguero
Jazyk: angličtina
Rok vydání: 2020
Předmět:
RNA viruses
0301 basic medicine
Viral Diseases
Physiology
medicine.medical_treatment
Virus Replication
Pathology and Laboratory Medicine
Mice
White Blood Cells
0302 clinical medicine
Animal Cells
Immune Physiology
Medicine and Health Sciences
Immune Response
RAW 264.7 Cells
Virus quantification
Innate Immune System
Chikungunya Virus
Multidisciplinary
biology
U937 cell
virus diseases
U937 Cells
Animal Models
Infectious Diseases
Cytokine
Experimental Organism Systems
Medical Microbiology
Viral Pathogens
Viruses
Disease Progression
Cytokines
Medicine
Tumor necrosis factor alpha
Cellular Types
Pathogens
medicine.symptom
Research Article
Neglected Tropical Diseases
Immune Cells
Alphaviruses
Science
Immunology
030231 tropical medicine
Mouse Models
Inflammation
Alphavirus
Research and Analysis Methods
Microbiology
Virus
Togaviruses
03 medical and health sciences
Model Organisms
Signs and Symptoms
Diagnostic Medicine
Virology
medicine
Animals
Humans
Animal Models of Disease
Microbial Pathogens
Blood Cells
Macrophages
Organisms
Biology and Life Sciences
Chikungunya Infection
Cell Biology
Molecular Development
Tropical Diseases
biology.organism_classification
Viral Replication
Animal Models of Infection
030104 developmental biology
Immune System
Animal Studies
Chikungunya Fever
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 3, p e0230328 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes rash, fever and severe polyarthritis that can last for years in humans. Murine models display inflammation and macrophage infiltration only in the adjacent tissues at the site of inoculation, showing no signs of systemic polyarthritis. Monocyte-derived macrophages are one cell type suspected to contribute to a systemic CHIKV infection. The purpose of this study was to analyze differences in CHIKV infection in two different cell lines, human U937 and murine RAW264.7 monocyte derived macrophages. PMA-differentiated U937 and RAW264.7 macrophages were infected with CHIKV, and infectious virus production was measured by plaque assay and by reverse transcriptase quantitative PCR at various time points. Secreted cytokines in the supernatants were measured using cytometric bead arrays. Cytokine mRNA levels were also measured to supplement expression data. Here we show that CHIKV replicates more efficiently in human macrophages compared to murine macrophages. In addition, infected human macrophages produced around 10-fold higher levels of infectious virus when compared to murine macrophages. Cytokine induction by CHIKV infection differed between human and murine macrophages; IL-1, IL-6, IFN-γ, and TNF were significantly upregulated in human macrophages. This evidence suggests that CHIKV replicates more efficiently and induces a much greater pro-inflammatory cytokine profile in human macrophages, when compared to murine macrophages. This may shed light on the critical role that macrophages play in the CHIKV inflammatory response.
Databáze: OpenAIRE
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