In situ evaluation of spatiotemporal distribution of doxorubicin from Drug-eluting Beads in a tissue mimicking phantom
| Autor: | Matthew R. Dreher, Pedro Garcia, Sean L. Willis, Andrew L. Lewis, Shuning Bian, Daniel F. Daly, Marcus Caine, Alexander Henman, Yiqing Tang, Robert Carlisle, Eleanor Stride |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
In situ
Materials science Pharmaceutical Science 02 engineering and technology Bead 030226 pharmacology & pharmacy Imaging phantom law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Confocal microscopy law In vivo Distribution (pharmacology) Chemoembolization Therapeutic Drug Carriers 021001 nanoscience & nanotechnology Microspheres Drug Liberation chemistry Doxorubicin visual_art visual_art.visual_art_medium Agarose 0210 nano-technology Drug carrier Biomedical engineering |
| DOI: | 10.1016/j.ejps.2021.105772 |
| Popis: | Understanding the intra-tumoral distribution of chemotherapeutic drugs is extremely important in predicting therapeutic outcome. Tissue mimicking gel phantoms are useful for studying drug distribution in vitro but quantifying distribution is laborious due to the need to section phantoms over the relevant time course and individually quantify drug elution. In this study we compare a bespoke version of the traditional phantom sectioning approach, with a novel confocal microscopy technique that enables dynamic in situ measurements of drug concentration. Release of doxorubicin from Drug-eluting Embolization Beads (DEBs) was measured in phantoms composed of alginate and agarose over comparable time intervals. Drug release from several different types of bead were measured. The non-radiopaque DC Bead™ generated a higher concentration at the boundary between the beads and the phantom and larger drug penetration distance within the release period, compared with the radiopaque DC Bead LUMI™. This is likely due to the difference of compositional and structural characteristics of the hydrogel beads interacting differently with the loaded drug. Comparison of in vitro results against historical in vivo data show good agreement in terms of drug penetration, when confounding factors such as geometry, elimination and bead chemistry were accounted for. Hence these methods have demonstrated potential for both bead and gel phantom validation, and provide opportunities for optimisation of bead design and embolization protocols through in vitro-in vivo comparison. |
| Databáze: | OpenAIRE |
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