Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus
| Autor: | Yi Hsiang Chen, Rei Lin Kuo, Chung Guei Huang, Li-Ang Lee, Sheng-Yu Huang, Shin-Ru Shih, Chun Yuan Lin, Chih Heng Huang, Ting-Wen Chen, Yueh Te Lin, Chi Jene Chen, Mei Feng Chen, Shu Ming Kuo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
viruses miR-1290 Vimentin virus Biology medicine.disease_cause viral ribonucleoprotein Virus Article 03 medical and health sciences 0302 clinical medicine vimentin Drug Discovery microRNA Influenza A virus medicine influenza A virus ferret Polymerase Ribonucleoprotein miRNA Gene knockdown lcsh:RM1-950 Cell biology vRNP 030104 developmental biology lcsh:Therapeutics. Pharmacology Viral replication host species-specificity 030220 oncology & carcinogenesis biology.protein Molecular Medicine |
| Zdroj: | Molecular Therapy. Nucleic Acids Molecular Therapy: Nucleic Acids, Vol 17, Iss, Pp 10-23 (2019) |
| ISSN: | 2162-2531 |
| Popis: | The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3′ UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication. Graphical Abstract |
| Databáze: | OpenAIRE |
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