Gene expression in human cumulus cells: one approach to oocyte competence
| Autor: | P. Feuerstein, Rozenn Dalbies-Tran, D. Royère, R. Bidault, F. Guerif, Véronique Cadoret |
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| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic [SDV]Life Sciences [q-bio] Cell Communication 0302 clinical medicine Gene expression reproductive and urinary physiology ComputingMilieux_MISCELLANEOUS Regulation of gene expression Genetics MATURATION OVOCYTAIRE 0303 health sciences 030219 obstetrics & reproductive medicine Cumulus Cells Reverse Transcriptase Polymerase Chain Reaction EXPRESSION DU GENE Steroidogenic acute regulatory protein Rehabilitation Obstetrics and Gynecology Gene Expression Regulation Developmental Embryo [SDV] Life Sciences [q-bio] Meiosis medicine.anatomical_structure Intercellular Signaling Peptides and Proteins Female Stearoyl-CoA Desaturase Adult endocrine system EGF Family of Proteins [INFO] Computer Science [cs] Biology Amphiregulin Andrology 03 medical and health sciences medicine Humans [INFO]Computer Science [cs] Blastocyst RNA Messenger Sperm Injections Intracytoplasmic CELLULE DE CUMULUS 030304 developmental biology Glycoproteins urogenital system Gene Expression Profiling Oocyte Phosphoproteins Gene expression profiling Reproductive Medicine Cyclooxygenase 2 Connexin 43 Oocytes |
| Zdroj: | Human Reproduction Human Reproduction, Oxford University Press (OUP), 2007, 22 (12), pp.3069-3077 HAL |
| ISSN: | 0268-1161 1460-2350 |
| Popis: | BACKGROUND: Dialogue between the oocyte and cumulus cells is essential for oocyte maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in cumulus cells harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according to individual oocyte nuclear maturity and developmental competence. Genes were chosen because their expression was induced by the LH peak [Steroidogenic Acute Regulatory protein (STAR), Cyclooxygenase 2 (COX2 or PTGS2), Amphiregulin (AREG)] or because they were involved in oocyte lipidic metabolism [Stearoyl-Coenzyme A Desaturase 1 and 5 (SCD1 and SCD5)] or in gap-junctions [Connexin 43 (CX43 or GJA1)]. METHODS: mRNA levels in cumulus cells were assessed by real-time PCR. RESULTS: Expression levels of all genes investigated, except Cx43, were increased after resumption of meiosis. Nuclear maturation was thus associated with increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells. When considering only cumulus associated with metaphase II oocytes, gene expression was independent of morphological status at Day 2. In contrast, transcript levels were lower and distributed over a narrower range in cumulus enclosing oocytes achieving blastocyst development at Day 5/6 than in cumulus enclosing oocytes unable to develop beyond the embryo stage. CONCLUSION: Further developmental potential from embryo to blastocyst stage was associated with lower expression in a narrow range for these genes. |
| Databáze: | OpenAIRE |
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