Transcription factor GATA-6 is expressed in the endocrine and GATA-4 in the exocrine pancreas

Autor: Hannele Niemi, Markku Heikinheimo, Mari-Anne Pulkkinen, Jaan Palgi, Ilkka Ketola, Timo Otonkoski, David B. Wilson, Christina Jacobsen
Rok vydání: 2004
Předmět:
Male
medicine.medical_specialty
Cellular differentiation
Enteroendocrine cell
In situ hybridization
Biology
Biochemistry
Immunoenzyme Techniques
Mice
03 medical and health sciences
Transcription Factor GATA-6
0302 clinical medicine
Endocrinology
Endocrine Glands
GATA6 Transcription Factor
Internal medicine
Gene expression
medicine
Animals
Endocrine system
RNA
Messenger
Molecular Biology
Cells
Cultured
In Situ Hybridization
030304 developmental biology
Homeodomain Proteins
0303 health sciences
Endoderm
Gene Expression Regulation
Developmental
Cell Differentiation
Pancreas
Exocrine
GATA4 Transcription Factor
Cell biology
DNA-Binding Proteins
medicine.anatomical_structure
030220 oncology & carcinogenesis
embryonic structures
Mice
Inbred CBA
Trans-Activators
Female
Pancreas
Transcription Factors
Endocrine gland
Zdroj: Molecular and Cellular Endocrinology. 226:51-57
ISSN: 0303-7207
Popis: GATA-4 and GATA-6 are zinc finger transcription factors that regulate gene expression, differentiation, and cell proliferation in various tissues. These factors have been implicated in the development of endodermal derivatives, including epithelial cells in the yolk sac, lung, and stomach. In the present study, we have characterized the expression of GATA-4 and GATA-6 during development of another endodermal derivative, the mouse pancreas, using a combination of in situ hybridization and immunohistochemistry. Neither GATA-4 nor GATA-6 antigen was detected in E10.5 pancreatic epithelial buds expressing Pdx-1. By E15.5, GATA-4 mRNA and protein were evident in developing pancreatic acini, but not in ductal or endocrine cells of the pancreas; GATA-6 mRNA and protein were present in both endocrine and exocrine cell precursors. In the newborn and adult pancreas, GATA-4 protein was seen in acinar cells, while GATA-6 antigen was found mainly in islet beta-cells. The amphicrine pancreatic AR42J-B13 cell line was used to study the expression of GATA-4 and GATA-6 during the differentiation of these cells towards an endocrine phenotype. Endocrine differentiation was associated with marked increase in GATA-6 but not GATA-4 mRNA levels. We conclude that GATA-4 is a marker of exocrine pancreatic differentiation, whereas GATA-6 is a marker of endocrine pancreatic development.
Databáze: OpenAIRE
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