Nanoparticles prepared from porcine cells support the healing of cutaneous inflammation in mice and wound re-epithelialization in human skin
| Autor: | K. Kalies, Franz Helbig, Melanie Barra, Sebastian Maass, Charles C. Caldwell, Kathrin Kalies, Stefan Soellner, Jürgen Westermann, Matthias Klinger, Lars Hecht, Eva Hauenschild, Natalia Kunz, Ralf Ludwig |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Epidermolysis bullosa acquisita Adult medicine.medical_specialty Swine Leishmaniasis Cutaneous Human skin Inflammation Dermatology Epidermolysis Bullosa Acquisita Biochemistry 03 medical and health sciences Mice Young Adult Th2 Cells Re-Epithelialization Cell-Derived Microparticles medicine Animals Humans Leishmania major Molecular Biology Interleukin 4 integumentary system biology business.industry Macrophages Cancer Cell Differentiation Middle Aged medicine.disease biology.organism_classification In vitro Mice Inbred C57BL 030104 developmental biology Immunology Female Interleukin-4 Lymph Nodes medicine.symptom Wound healing business |
| Zdroj: | Experimental dermatology. 26(12) |
| ISSN: | 1600-0625 |
| Popis: | Previous reports have demonstrated that cell-derived nanoparticles (CDNPs) composed of bovine or porcine protein complexes exerted therapeutic effects against viral infections and cancer in mice and humans. Based on these observations, we asked whether CDNPs would improve inflammatory skin disorders. To address this, we utilized two distinct mouse models of cutaneous inflammation: the autoimmune skin-blistering disease epidermolysis bullosa acquisita (EBA) as an example of an autoantibody-induced cutaneous inflammation, and Leishmania major (L. major) infection as an example of a pathogen-induced cutaneous inflammation. In both models, we observed that CDNPs increased mRNA expression of the Th2 cytokine IL-4. Clinically, CDNPs decreased inflammation due to EBA and increased L. major-specific IgG1 levels without major effects on infected skin lesions. In addition, CDNPs supported the growth of keratinocytes in human skin cultures. In vitro studies revealed that CDNPs were taken up predominantly by macrophages, leading to a shift towards the expression of anti-inflammatory cytokine genes. Altogether, our data demonstrate that treatment with porcine CDNPs may be a new therapeutic option for the control of autoimmune-mediated inflammatory skin disorders. |
| Databáze: | OpenAIRE |
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