The Plasma Membrane is Involved in the Visible Light–Tissue Interaction
| Autor: | Ronit Lavi, Michael S. Sinyakov, Rinat Ankri, Asher Shainberg, Rachel Lubart, Haim Breitbart, Maor Eichler, H. Friedmann |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Light Radical Biomedical Engineering Photochemistry law.invention Cyclic N-Oxides chemistry.chemical_compound law Animals Radiology Nuclear Medicine and imaging Photosensitizer Electron paramagnetic resonance chemistry.chemical_classification Reactive oxygen species Sheep NADPH oxidase Spin trapping biology Cell Membrane Electron Spin Resonance Spectroscopy NADPH Oxidases Spermatozoa Membrane chemistry biology.protein Reactive Oxygen Species Spin Trapping Nicotinamide adenine dinucleotide phosphate |
| Zdroj: | Photomedicine and Laser Surgery. 30:14-19 |
| ISSN: | 1557-8550 1549-5418 |
| DOI: | 10.1089/pho.2011.3083 |
| Popis: | The aim of the present study was to determine whether the plasma membrane is also involved in the light-tissue interaction because of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase electron chain, which can serve as a photosensitizer.It has been suggested that the mechanism of photobiostimulation involves light-induced low levels of reactive oxygen species (ROS) that serve as signal transduction messengers. Production of ROS following visible-light irradiation was verified by the electron paramagnetic resonance (EPR) spin-trapping technique, and the mitochondrial cytochromes were suggested as the main cellular target for visible-light absorption.Isolated sperm membranes were illuminated with visible light and the increase in oxygen radical production was measured using the EPR spin-trapping technique coupled with the probe 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). A broadband visible light source (400-800 nm) at 40-130 mW/cm(2) with appropriate filters provided the illumination. In order to determine whether the light-induced ROS production is a result of a photo-accelerated electron transfer in the enzyme-catalyzed reaction with oxygen in the plasma membrane, or resulted from a photochemical reaction of the chromophores alone with oxygen, denatured membranes were irradiated as well.Visible-light-induced oxyradicals were detected in isolated sperm membranes. Blue light was found to be more effective than red. Illuminated denatured membranes produced the same amount of ROS as non-denatured membranes.Visible-light illumination, especially in the blue region, increases ROS levels in isolated plasma membranes. The mechanism of ROS formation is probably a photochemical reaction of the membranal chromophhores, for example, cytochromes or flavins with oxygen, and not an enzyme-catalyzed photochemical reaction. |
| Databáze: | OpenAIRE |
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