Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease

Autor: Jiandong Yang, Yoshikazu Hirai, Kei Iida, Shinji Ito, Marika Trumm, Shiho Terada, Risako Sakai, Toshiyuki Tsuchiya, Osamu Tabata, Ken-ichiro Kamei
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Communications Biology. 6
ISSN: 2399-3642
Popis: Non-alcoholic fatty liver disease (NAFLD) afflicts a significant percentage of the population; however, no effective treatments have yet been established because of the unsuitability of in vitro assays and animal experimental models. Here, we present an integrated-gut-liver-on-a-chip (iGLC) platform as an in vitro human model of the gut-liver axis (GLA) by co-culturing human gut and liver cell lines interconnected via microfluidics in a closed circulation loop, for the initiation and progression of NAFLD by treatment with free fatty acids (FFAs) for 1 and 7 days, respectively. Co-cultured Caco-2 gut-mimicking cells and HepG2 hepatocyte-like cells demonstrate the protective effects from apoptosis against FFAs treatment, whereas mono-cultured cells exhibit induced apoptosis. Phenotype and gene expression analyses reveal that the FFAs-treated gut and liver cells accumulated intracellular lipid droplets and show an increase in gene expression associated with a cellular response to copper ions and endoplasmic reticulum stress. As an in vitro human GLA model, the iGLC platform may serve as an alternative to animal experiments for investigating the mechanisms of NAFLD.
非アルコール性脂肪性肝疾患を再現した腸・肝連結臓器チップの開発. 京都大学プレスリリース. 2023-04-07.
Two-organ chip to answer fatty liver questions. 京都大学プレスリリース. 2023-04-07.
Databáze: OpenAIRE
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