Increased responsiveness to TLR2 and TLR4 ligands during dimethylsulfoxide-induced neutrophil-like differentiation of HL-60 myeloid leukemia cells
| Autor: | Yuko Ohira, Takashi Furuta, Dieter C. Gruenert, Tsuyoshi Shuto, Mary Ann Suico, Keizo Sato, Judy Cheung, Hirofumi Kai, Shogo Shimasaki |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Lipopolysaccharide Neutrophils CD14 Lipopolysaccharide Receptors Lymphocyte Antigen 96 HL-60 Cells Ligands Proinflammatory cytokine chemistry.chemical_compound Gene expression Humans Dimethyl Sulfoxide Toll-like receptor Chemistry Myeloid leukemia Cell Differentiation Hematology Toll-Like Receptor 2 Up-Regulation Cell biology Toll-Like Receptor 4 TLR2 Oncology Leukemia Myeloid Cancer research TLR4 lipids (amino acids peptides and proteins) |
| Zdroj: | Leukemia Research. 31:1721-1728 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2007.06.011 |
| Popis: | Neutrophils express functional toll-like receptor2 (TLR2) and 4 (TLR4) that are crucial for the production of inflammatory cytokines. Here, we show that dimethylsulfoxide-induced neutrophil-like differentiation of promyelocytic HL-60 cells (dHL-60) results in cells that respond to TLR2 and TLR4 ligands similarly to primary neutrophils. Consistent with the increased responsiveness of the cells to TLR2 ligand, the TLR2 gene was strongly up-regulated in dHL-60 cells. On the other hand, increased surface expression of LPS receptor complex, TLR4/MD2/CD14, was observed without affecting TLR4 gene expression. Thus, the data demonstrate a higher responsiveness of dHL-60 cells to TLR2 and TLR4 ligands because of increased TLR2 and MD2/CD14 gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect