Alkaloid production in Catharanthus roseus cell cultures: Initial studies on cell lines and their alkaloid content
| Autor: | Brian R. Worth, James P Kutney, Lewis S L Choi, Stephen K. Sleigh, Friedrich Constabel, K. B. Chatson, Pawel Kolodziejczyk, Wolfgang G. W. Kurz, Kenneth L. Stuart |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
cell culture
endocrine system Catharanthus roseus Apocynaceae variability organic chemicals Alkaloid Strychnos Plant Science General Medicine Horticulture Biology biology.organism_classification complex mixtures Biochemistry Corynanthe Tissue culture Dry weight indole alkaloids Botany Aspidosperma heterocyclic compounds Molecular Biology |
| Zdroj: | Phytochemistry. 19:2583-2587 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/s0031-9422(00)83923-0 |
| Popis: | Several hundred serially cultured cell suspensions derived from three cultivars of periwinkle ( Catharanthus roseus ) were established in Gamborg's B 5 medium and then transferred to Zenk's alkaloid production medium. Total alkaloid concentration ranged from 0.1 to 1.5% of dry weight. Alkaloids present were of the corynanthe, strychnos and aspidosperma types, with the greatest diversity arising during the third to the fifth week of subculturing. The alkaloid content appeared both specific for, and reproducible in, individual cell lines. |
| Databáze: | OpenAIRE |
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