| Autor: |
Bargar, Connor, De Luca, Chiara Maria Giulia, Devigili, Grazia, Elia, Antonio Emanuele, Cilia, Roberto, Portaleone, Sara Maria, Wang, Wen, Tramacere, Irene, Bistaffa, Edoardo, Cazzaniga, Federico Angelo, Felisati, Giovanni, Legname, Giuseppe, Di Fonzo, Alessio, Xu, Rong, Gunzler, Steven Alexander, Giaccone, Giorgio, Eleopra, Roberto, Chen, Shu Guang, Moda, Fabio |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.17163347.v1 |
| Popis: |
Additional file 4. Morphological analsysis of ��Syn_RT-QuIC reaction products generated by MSA-P and MSA-C samples at ITA-lab. ��Syn_RT-QuIC reaction products were properly diluted and 10 ��L of the final dilutions was dropped onto 200-mesh Formvar-carbon coated nickel grids for 30 min and the remaining drop was blotted dry using filter papers. Samples were stained with 25% Uranyl Acetate Replacement (UAR, negative staining) for 10 min, the solution was removed using filter papers and the grids were air-dried for 15 min before the analyses. Images were recorded at 120 kV with a FEI Tecnai Spirit, equipped with an Olimpus Megaview G2 camera. Rec-��Syn amyloid fibrils were detected in all ��Syn_RT-QuIC products seeded with MSA-P samples, except for MSA-P_10 and MSA-P_11. In constrast, none of the MSA-C samples induced the formation of rec-��Syn fibrils (except for occasional, rare and short aggregates detected in MSA-C_1, MSA-C_3 and MSA-C_6). All TEM images were taken at the same magnification (scale bar: 200 nm). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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