Targeting of tail-anchored proteins to yeast mitochondria in vivo
| Autor: | Sandra Isenmann, Traude H. Beilharz, Rebecca George, Sabine Gratzer, Trevor Lithgow, Binks W. Wattenberg, Billie Egan |
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| Rok vydání: | 1999 |
| Předmět: |
Signal peptide
Translocase of the outer membrane Molecular Sequence Data Biophysics Saccharomyces cerevisiae medicine.disease_cause Biochemistry Transmembrane domain Fungal Proteins Mitochondrial membrane transport protein Tail anchor Structural Biology Protein targeting Genetics medicine Amino Acid Sequence Mitochondrion Molecular Biology Integral membrane protein Protein translocation Binding Sites biology Membrane Proteins Biological Transport Cell Biology Transmembrane protein Cell biology Mitochondria Membrane protein Translocase of the inner membrane biology.protein Carrier Proteins |
| Zdroj: | FEBS letters. 451(3) |
| ISSN: | 0014-5793 |
| Popis: | Tail-anchored proteins are inserted into intracellular membranes via a C-terminal transmembrane domain. The topology of the protein is such that insertion must occur post-translationally, since the insertion sequence is not available for membrane insertion until after translation of the tail-anchored polypeptide is completed. Here, we show that the targeting information in one such tail-anchored protein, translocase in the outer mitochondrial membrane 22, is contained in a short region flanking the transmembrane domain. An equivalent region is sufficient to specify the localisation of Bcl2 and SNARE proteins to the secretory membranes. We discuss the targeting process for directing members of this protein family to the secretory and mitochondrial membranes in vivo. |
| Databáze: | OpenAIRE |
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