Draft genome of the brown alga, Nemacystus decipiens, Onna-1 strain: Fusion of genes involved in the sulfated fucan biosynthesis pathway
| Autor: | Asuka Arimoto, Eiichi Shoguchi, Koki Nishitsuji, Yoshimi Higa, Mayumi Kawamitsu, Munekazu Mekaru, Noriyuki Satoh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA Hydrolases lcsh:Medicine Phaeophyta Genome Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Japan Polysaccharides lcsh:Science Gene Genetics Multidisciplinary biology Fucoidan Ectocarpus siliculosus lcsh:R Cladosiphon okamuranus Molecular Sequence Annotation Hydrolase Gene biology.organism_classification Seaweed Biosynthetic Pathways Brown algae 030104 developmental biology chemistry lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2045-2322 |
| Popis: | The brown alga, Nemacystus decipiens (“ito-mozuku” in Japanese), is one of the major edible seaweeds, cultivated principally in Okinawa, Japan. N. decipiens is also a significant source of fucoidan, which has various physiological activities. To facilitate brown algal studies, we decoded the ~154 Mbp draft genome of N. decipiens Onna-1 strain. The genome is estimated to contain 15,156 protein-coding genes, ~78% of which are substantiated by corresponding mRNAs. Mitochondrial genes analysis showed a close relationship between N. decipiens and Cladosiphon okamuranus. Comparisons with the C. okamuranus and Ectocarpus siliculosus genomes identified a set of N. decipiens-specific genes. Gene ontology annotation showed more than half of these are classified as molecular function, enzymatic activity, and/or biological process. Extracellular matrix analysis revealed domains shared among three brown algae. Characterization of genes that encode enzymes involved in the biosynthetic pathway for sulfated fucan showed two sets of genes fused in the genome. One is a fusion of l-fucokinase and GDP-fucose pyrophosphorylase genes, a feature shared with C. okamuranus. Another fusion is between an ST-domain-containing gene and an alpha/beta hydrolase gene. Although the function of fused genes should be examined in future, these results suggest that N. decipiens is another promising source of fucoidan. |
| Databáze: | OpenAIRE |
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