Quantitative Clonal Analysis and Single-Cell Transcriptomics Reveal Division Kinetics, Hierarchy, and Fate of Oral Epithelial Progenitor Cells
| Autor: | Hongfang Ma, Ophir D. Klein, Pauline Marangoni, Sachiko Furukawa, Rebecca S. D’Urso, Rapolas Zilionis, Kyle B. Jones, Henry Pinkard, Allon M. Klein |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Cell division Kinetics Biology Clonal analysis Article 03 medical and health sciences Basal (phylogenetics) Mice 0302 clinical medicine Proto-Oncogene Proteins Genetics medicine Animals Homeostasis Cell Lineage Progenitor cell Oral mucosa 030304 developmental biology Mice Knockout Polycomb Repressive Complex 1 0303 health sciences Stem Cells Mouth Mucosa Cell Differentiation Epithelial Cells Cell Biology Cell biology Mice Inbred C57BL medicine.anatomical_structure Molecular Medicine Female Stem cell Single-Cell Analysis Transcriptome 030217 neurology & neurosurgery Cell Division |
| Zdroj: | Cell stem cell. 24(1) |
| ISSN: | 1875-9777 |
| Popis: | Summary The oral mucosa is one of the most rapidly dividing tissues in the body and serves as a barrier to physical and chemical insults from mastication, food, and microorganisms. Breakdown of this barrier can lead to significant morbidity and potentially life-threatening infections for patients. Determining the identity and organization of oral epithelial progenitor cells (OEPCs) is therefore paramount to understanding their roles in homeostasis and disease. Using lineage tracing and label retention experiments, we show that rapidly dividing OEPCs are located broadly within the basal layer of the mucosa throughout the oral cavity. Quantitative clonal analysis demonstrated that OEPCs undergo population-asymmetrical divisions following neutral drift dynamics and that they respond to chemotherapy-induced damage by altering daughter cell fates. Finally, using single-cell RNA-seq, we establish the basal layer population structure and propose a model that defines the organization of cells within the basal layer. |
| Databáze: | OpenAIRE |
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