Decreased mitochondrial creatine kinase activity in dystrophic chicken breast muscle alters creatine-linked respiratory coupling
| Autor: | Clarence H. Suelter, Vickie D. Bennett, Norman Hall, Marlene DeLuca |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
Time Factors Phosphocreatine Biophysics In Vitro Techniques Mitochondrion Reductase Creatine Biochemistry Mitochondria Heart Oxidative Phosphorylation Phosphates chemistry.chemical_compound Adenosine Triphosphate Internal medicine Respiration medicine Animals Respiratory system skin and connective tissue diseases Inner mitochondrial membrane Creatine Kinase Molecular Biology biology Muscular Dystrophy Animal Mitochondria Muscle Coupling (electronics) Endocrinology chemistry biology.protein Creatine kinase Chickens |
| Zdroj: | Archives of Biochemistry and Biophysics. 240:380-391 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(85)90043-8 |
| Popis: | Dystrophic chicken breast muscle mitochondria contain significantly less mitochondrial creatine kinase than normal breast muscle mitochondria. Breast muscle mitochondria from normal 16- to 40-day-old chickens contain approximately 80 units of mitochondrial creatine kinase per unit of succinate:INT ( p -iodonitrotetrazolium violet) reductase, a mitochondrial marker, while dystrophic chicken breast muscle mitochondria contain 36–44 units. Normal chicken heart muscle mitochondria contain about 10% of the mitochondrial creatine kinase per unit of succinate:INT reductase as normal breast muscle mitochondria. The levels in heart muscle mitochondria from dystrophic chickens are not affected significantly. Evidence is presented which shows that the reduced level of mitochondrial creatine kinase in dystrophic breast muscle mitochondria is responsible for an altered creatine linked respiration. First, both normal and dystrophic breast muscle mitochondria respire with the same state 3 and state 4 respiration. Second, the post-ADP state 4 rate of respiration of normal breast muscle mitochondria in the presence of 20 m m creatine continues at the state 3 rate. However, the state 4 rate of dystrophic breast muscle mitochondria and mitochondria from other muscle types with a low level of mitochondrial creatine kinase, such as heart muscle and 5-day-old chicken breast muscle, is slower than the state 3 rate. Third, dystrophic breast mitochondria synthesize ATP at the same rate as normal breast muscle mitochondria but rates of creatine phosphate synthesis in 20–50 m m P i are reduced significantly. Finally, increasing concentrations of P i displace mitochondrial creatine kinase from mitoplasts of normal and dystrophic breast muscle mitochondria with the same apparent K D , indicating that the outer surface of the inner mitochondrial membrane and the mitochondrial creatine kinase from dystrophic muscle are not altered. |
| Databáze: | OpenAIRE |
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