Evaluation of functional SiO2 nanoparticles toxicity by a 3D culture model
| Autor: | Sylvie Jeanne, Sylvie Tricot-Doleux, Fabien Grasset, Stéphane Cordier, Francisco Cabello-Hurtado, Nicolas Nerambourg, Pascal Pellen-Mussi, Chrystelle Neaime |
|---|---|
| Přispěvatelé: | Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), CLUSTOP 2011 BS08 013 01, ANR, Agence Nationale de la Recherche, CNRS, Centre National de la Recherche Scientifique, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Cell viability
Materials science Biomedical Engineering Nanoparticle Bioengineering Context (language use) 02 engineering and technology 010402 general chemistry 01 natural sciences Clusters General Materials Science Viability assay Cytotoxicity ComputingMilieux_MISCELLANEOUS General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 3. Good health 0104 chemical sciences Colloidal gold Cell culture Nanotoxicology Oxidative stress Toxicity Biophysics [SDE.BE]Environmental Sciences/Biodiversity and Ecology 0210 nano-technology |
| Zdroj: | Journal of Nanoscience and Nanotechnology Journal of Nanoscience and Nanotechnology, American Scientific Publishers, 2018, 18 (5), pp.3148-3157. ⟨10.1166/jnn.2018.14619⟩ Journal of Nanoscience and Nanotechnology, 2018, 18 (5), pp.3148-3157. ⟨10.1166/jnn.2018.14619⟩ |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2018.14619⟩ |
| Popis: | Context as a kind of non-metal oxide SiO2 NPs have been extensively used in biomedicine, pharmaceuticals and other industrial manufacturing fields, such as DNA delivery, cancer therapy… Our group had developed a method based on microemulsion process to prepare SiO2 NPs incorporating photonic or magnetic nanocrystals and luminescent nanosized inorganic metal atom clusters. However, the toxicity of nanoparticles is known to be closely related to their physico-chemical characteristics and chemical composition. Object it is therefore of interest to investigate the toxicity of these novel SiO2 NPs to the cells that may come in contact. Materials and methods the potential toxic effect of the functional @SiO2 NPs containing Mo6 clusters with or without gold nanoparticles was investigated, at concentrations 1 μg/mL, 10 μg/mL and 100 μg/mL each, on three different cell lines. Cell viability was measured by the MTT test in monolayer's culture whereas the cytotoxicity in spheroid model was examined by the APH assay. In a second time, oxidative-stress-induced cytotoxicity was investigated through glutathione levels dosages. Results the results indicated that both A549 and L929 cell lines did not exhibit susceptibility to functional @SiO2 NPs-induced oxidative stress unlike KB cells. Discussion SiO2 NPs containing CMB may become toxic to cultured cells but only at a very high dosage level. Therefore, this toxicity depends on cell lines and more, on the model of cell cultures. The selection of appropriate cell line remains a critical component in nanotoxicology. Conclusion these results are relevant to future applications of SiO2 gold-cluster NPs in controlled release applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|