Derivatization-free LC-MS/MS method for estrogen quantification in mouse brain highlights a local metabolic regulation after oral versus subcutaneous administration
| Autor: | Mylène Potier, Shaam Alice Al Abed, Valérie Lamothe, Svitlana Shinkaruk, Corinne Buré, Jean-Marie Schmitter, Ecaterina Lozan, Aline Marighetto, Catherine Bennetau-Pelissero |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Time Factors medicine.drug_class Metabolite medicine.medical_treatment Central nervous system Administration Oral Estrone Pharmacology Hippocampus Biochemistry Chemistry Techniques Analytical Analytical Chemistry Steroid Mice 03 medical and health sciences chemistry.chemical_compound Subcutaneous injection 0302 clinical medicine Subcutaneous Absorption Tandem Mass Spectrometry medicine Animals Brain Chemistry Solid Phase Extraction Estrogens Estriol 030104 developmental biology medicine.anatomical_structure chemistry Estrogen 030217 neurology & neurosurgery Chromatography Liquid Hormone |
| Popis: | 17β-Estradiol (17β-E2) is a steroid with pleiotropic actions. In addition to being a sexual hormone, it is also produced in the brain where it modulates the reproductive axis. It has been shown that 17β-E2 also acts on synaptic plasticity and plays a role in neurological pathways and in neurodegenerative diseases. Assaying this steroid in the brain is thus interesting to improve our knowledge of 17β-E2 effects in the brain. However, 17β-E2 concentration in the central nervous system has been reported to be of a few nanograms per gram wet weight (nanomolar range concentration); therefore, its quantification requires both an efficient extraction process and a sensitive detection method. Herein is presented a derivatization-free procedure based on solid-phase extraction followed by LC-MS/MS analysis, targeted on 17β-E2, its isomer17α-E2, and its metabolites estrone (E1) and estriol (E3). This extraction process allowed reaching 96% 17β-E2 recovery from the mouse brain. Limit of detection (LOD) and limit of quantification (LOQ) values of 0.5 and 2.5 pmol mL−1, respectively, were reached for both 17α-E2 and 17β-E2. LOD values for E1 and E3 were 0.01 and 0.025 pmol mL−1, respectively. The variation coefficients for intra- and inter-assays were 6 and 14%, respectively, for both estradiol forms. The method was applied to assess estrogen levels in the mouse brain and hippocampus after 17β-E2 acute (subcutaneous injection) and chronic (drinking water) physiological administration. Total estrogen levels were determined after enzymatic deconjugation and compared to free estrogen levels. While 17α-E2 was not detected in biological samples, 17β-E2 and metabolite measurements highlight a local biotransformation of estrogens after physiological administration via drinking water. |
| Databáze: | OpenAIRE |
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