Evidence that endogenous hydrogen sulfide exerts an excitatory effect on gastric motility in mice
| Autor: | Wen-Xie Xu, Young Chul Kim, Hong-Li Lu, Xin Guo, Dong-Hai Liu, Xu Huang, Yi-Song Wu, Yan-Fei Han |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Patch-Clamp Techniques Blotting Western Myocytes Smooth Muscle Gastric motility Cystathionine beta-Synthase Sulfides Membrane Potentials chemistry.chemical_compound Mice Internal medicine medicine Myocyte Animals Patch clamp Hydrogen Sulfide Pharmacology Membrane potential Mice Inbred ICR Tetraethylammonium Dose-Response Relationship Drug Cystathionine gamma-Lyase Depolarization Aminooxyacetic acid Immunohistochemistry Endocrinology chemistry Excitatory postsynaptic potential Gastrointestinal Motility Delayed Rectifier Potassium Channels |
| Zdroj: | European journal of pharmacology. 673(1-3) |
| ISSN: | 1879-0712 |
| Popis: | The present study was designed to investigate the effect of endogenous hydrogen sulfide (H₂S) on gastric motility in mice. Western blotting and immunocytochemistry were used to determine expression levels of the H₂S-producing enzymes cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) in gastric tissues and cultured smooth muscle cells. Physiological and intracellular recordings and the whole-cell patch clamp technique were used to evaluate the effect of H₂S on the mechanical and electrical activities in muscle strips and in isolated smooth muscle cells, respectively. The results showed that CBS and CSE were expressed in mouse gastric smooth muscle. NaHS, a H₂S donor, inhibited the amplitude and frequency of spontaneous contraction at high concentrations (>200 μM). However, NaHS at low concentrations ( |
| Databáze: | OpenAIRE |
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