Oral fluid cocaine and benzoylecgonine concentrations following controlled intravenous cocaine administration
| Autor: | Kayla N. Ellefsen, Marta Concheiro, Marilyn A. Huestis, Sandrine Pirard, David A. Gorelick |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Narcotics Saliva Time Factors Metabolite Pharmacology 030226 pharmacology & pharmacy 01 natural sciences Gas Chromatography-Mass Spectrometry Article Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cocaine Pharmacokinetics Intravenous cocaine Humans Medicine business.industry 010401 analytical chemistry Half-life Middle Aged 0104 chemical sciences Substance Abuse Detection chemistry Injections Intravenous Benzoylecgonine Oral fluid Female business Law Half-Life |
| Zdroj: | Forensic Science International. 260:95-101 |
| ISSN: | 0379-0738 |
| DOI: | 10.1016/j.forsciint.2016.01.013 |
| Popis: | Limited oral fluid (OF) pharmacokinetic data collected with commercially available collection devices after controlled cocaine administration hinder OF result interpretations. Ten cocaine-using adults provided OF, collected with Oral-Eze ® (OE) and StatSure Saliva Sampler™ (SS) devices, an hour prior to and up to 69h after 25mg intravenous (IV) cocaine administration. Cocaine and benzoylecgonine (BE) were quantified by a validated 2D-GC-MS method. Large inter-subject variability was observed. Cocaine was detected in OF in the first 0.17h sample after IV administration, with much more rapid elimination than BE. OE observed C max median (range) concentrations were 932 (394–1574)μg/L for cocaine and 248 (96.9–953)μg/L for BE. SS observed cocaine and BE C max median (range) concentrations trended lower at 732 (83.3–1892)μg/L and 360 (77.2–836)μg/L, respectively. OE and SS cocaine OF detection times were 12.5 and 6.5h and for BE 30.5 and 28.0h, respectively at 1μg/L. There were no significant pharmacokinetic differences between OE and SS OF collection devices, except cocaine half-life was significantly shorter in SS OF specimens. This difference could be attributed to differences in stabilizing buffers present in OF collection devices, which may affect cocaine stability in OF specimens, or decreased recovery from collection pads. Both OE and SS OF collection devices were effective in monitoring cocaine and metabolite concentrations with similar detection windows. Furthermore, we demonstrated that different confirmatory OF cutoffs can be selected to produce shorter or longer cocaine and metabolite detection windows to address specific needs of clinical and forensic drug testing programs. |
| Databáze: | OpenAIRE |
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