Molecular analysis of zinc transporters in Schistosoma japonicum
| Autor: | Geoffrey N. Gobert, Sujeevi Nawaratna, Amber Glanfield, Leigh Schulte, Donald P. McManus, Malcolm K. Jones |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
DNA Complementary Iron Snails Immunology In situ hybridization Genome Schistosoma japonicum Mice Animals Amino Acid Sequence Cation Transport Proteins Gene In Situ Hybridization Phylogeny Schistosoma Base Sequence biology Transporter Helminth Proteins General Medicine DNA Helminth biology.organism_classification Molecular biology Zinc Infectious Diseases Real-time polymerase chain reaction Female Parasitology RNA Helminth Trematoda human activities |
| Zdroj: | Experimental Parasitology. 127:768-776 |
| ISSN: | 0014-4894 |
| Popis: | Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantitative PCR analyses of these ZIPs through the lifecycle showed that each is expressed predominantly during the intramammalian stage and are particularly enriched in adult females. Using laser microdissected tissue as template, SjZIPA-D were transcriptionally enriched in female reproductive tissues, SjZIPE was not expressed in specific tissues and SjZIPF was expressed similarly in each tissue. Whole mount in situ hybridization revealed that SjZIPA and SjZIPB were localised to the oesophageal gland of adults and the vitellaria. We have shown that multiple ZIPs are expressed by schistosomes during the intramammalian parasitic phases and propose that the encoded products perform diverse cellular functions related to metal transport in different cells of S. japonicum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect