Measurement of S100B protein: evaluation of a new prototype on a bioMérieux Vidas® 3 analyzer
| Autor: | Charlotte Oris, Damien Bouvier, Marina Brailova, Samy Kahouadji, Julie Durif, Russel Chabanne, Vincent Sapin |
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| Přispěvatelé: | CHU Clermont-Ferrand, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), BLANCHON, LOIC |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Clinical Biochemistry Computed tomography Roche Diagnostics Cobas S100B Automation 0302 clinical medicine MESH: Automation 030212 general & internal medicine [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Immunoassay MESH: Aged Vidas MESH: Middle Aged medicine.diagnostic_test MESH: Blood Chemical Analysis sample stability General Medicine Middle Aged 3. Good health Cost savings MESH: Young Adult Female MESH: Immunoassay Adult Spectrum analyzer S100 Calcium Binding Protein beta Subunit Young Adult 03 medical and health sciences 030225 pediatrics medicine Humans [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] S100b protein Aged Chromatography MESH: Humans business.industry MESH: S100 Calcium Binding Protein beta Subunit Biochemistry (medical) MESH: Adult MESH: Male Radiation exposure assay comparison Clinical diagnosis business MESH: Female Blood Chemical Analysis |
| Zdroj: | Clinical Chemistry and Laboratory Medicine Clinical Chemistry and Laboratory Medicine, 2019, 57 (8), pp.1177-1184. ⟨10.1515/cclm-2018-1217⟩ Clinical Chemistry and Laboratory Medicine, De Gruyter, 2019, 57 (8), pp.1177-1184. ⟨10.1515/cclm-2018-1217⟩ |
| ISSN: | 1434-6621 1437-4331 |
| DOI: | 10.1515/cclm-2018-1217⟩ |
| Popis: | Background The addition of S100B protein to guidelines for the management of mild traumatic brain injury (mTBI) decreases the amount of unnecessary computed tomography (CT) scans with a significant decrease in radiation exposure and an increase in cost savings. Both DiaSorin and Roche Diagnostics have developed automated assays for S100B determination. Recently, bioMérieux developed a prototype immunoassay for serum S100B determination. For the first time, we present the evaluation of the S100B measurement using a bioMérieux Vidas® 3 analyzer. Methods We evaluated the matrix effects of serum and plasma, and their stability after storage at 2–8 °C, −20 °C and −80 °C. The new measurement prototype (bioMérieux) was compared with an established one (Roche Diagnostics), and a precision study was also conducted. Lastly, clinical diagnostics performance of the bioMérieux and Roche Diagnostics methods were compared for 80 patients referred to the Emergency Department for mTBI. Results Stability after storage at 2–8 °C, −20 °C, and −80 °C and validation of the serum matrix were demonstrated. The bioMérieux analyzer was compared to the Roche Diagnostics system, and the analytical precision was found to be efficient. Clinical diagnosis performance evaluation confirmed the predictive negative value of S100B in the management of mTBI. Conclusions The study’s data are useful for interpreting serum S100B results on a bioMérieux Vidas® 3 analyzer. |
| Databáze: | OpenAIRE |
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