The Effect of ProHydrolase® on the Amino Acid and Intramuscular Anabolic Signaling Response to Resistance Exercise in Trained Males
| Autor: | Trisha A. VanDusseldorp, Stephen H. Heffington, Gerald T. Mangine, Claudia M. Boot, Megan D. Jones, Jaclyn E. Morimune, Jeremy R. Townsend, Autumn C. Marshall, Allison A. Haase, Yuri Feito, Cheryle N Beuning, Ruth N. Henry, Laurel A. Littlefield |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Whey protein Anabolism protein synthesis muscle medicine.medical_treatment education chemical and pharmacologic phenomena Physical Therapy Sports Therapy and Rehabilitation Article lcsh:GV557-1198.995 03 medical and health sciences 0302 clinical medicine immune system diseases Internal medicine medicine Orthopedics and Sports Medicine Leg press Essential amino acid lcsh:Sports chemistry.chemical_classification 030109 nutrition & dietetics Protease technology industry and agriculture Area under the curve hemic and immune systems protease 030229 sport sciences Amino acid Endocrinology chemistry Leucine leucine protein supplementation |
| Zdroj: | Sports Sports, Vol 8, Iss 2, p 13 (2020) Volume 8 Issue 2 |
| ISSN: | 2075-4663 |
| Popis: | This double-blind study examined effects of a protease enzyme blend (Prohydrolase® ) added to whey protein on post-resistance exercise aminoacidemia and intramuscular anabolic signaling were investigated in ten resistance-trained males. Participants completed 4 sets of 8&ndash 10 repetitions in the leg press and leg extension exercises at 75% of 1-repetition maximum. Participants then consumed either 250 mg of Prohydrolase® + 26 g of whey protein (PW), 26 g whey alone (W), or non-nutritive control (CON) in counterbalanced order. Blood samples were obtained prior to exercise (baseline) and then immediately-post (IP), 30-, 60-, 90-, 120-, and 180-min post-exercise. Muscle biopsies were taken at baseline, 1-h (1H), and 3-h (3H) post-exercise. Phosphorylation of AKTSer437 was decreased (3H only: p < 0.001), mTORSer2448 was increased (1H: p = 0.025 3H: p = 0.009), and p70S6KThr412 remained unchanged similarly for each condition. Plasma leucine, branch-chained amino acids, and essential amino acid concentrations for PW were significantly higher than CON (p < 0.05) at 30 min and similar to W. Compared to IP, PW was the only treatment with elevated plasma leucine levels at 30 min (p = 0.007 ∆ = 57.8 mmol/L, 95% Confidence Interval (CI): 20.0, 95.6) and EAA levels at 180 min (p = 0.003 ∆ = 179.1 mmol/L, 95% CI: 77.5, 280.7). Area under the curve amino acid analysis revealed no differences between PW and W. While no different than W, these data indicate that PW was the only group to produce elevated amino acid concentrations 30-min and 180-min post-ingestion. |
| Databáze: | OpenAIRE |
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