Characterization of Carbapenemase-Producing Enterobacteriaceae from Patients in Amman, Jordan
| Autor: | Katie L. Hopkins, Mohammad Al-Maayteh, Amin A. Aqel, Matthew J. Ellington, Awatef Al-Kaabneh, Hamed Alzoubi, Ibrahim Masalha, Neil Woodford, Jacqueline Findlay, Jane F. Turton |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Klebsiella pneumoniae 030106 microbiology Immunology Microbial Sensitivity Tests Carbapenem-resistant enterobacteriaceae Microbiology beta-Lactamases 03 medical and health sciences Plasmid Bacterial Proteins Humans Typing Pharmacology Jordan biology Enterobacteriaceae Infections biology.organism_classification Enterobacteriaceae Hospitals Anti-Bacterial Agents Variable number tandem repeat Carbapenem-Resistant Enterobacteriaceae Carbapenems Multilocus sequence typing Restriction fragment length polymorphism Multilocus Sequence Typing Plasmids |
| Zdroj: | Microbial Drug Resistance. 24:1121-1127 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2017.0238 |
| Popis: | We sought to detect and determine the genetic diversity of carbapenemase-producing Enterobacteriaceae (CPE) isolated from clinical specimens in Amman, Jordan. From five hospitals, a total of 2,759 isolates had antimicrobial susceptibilities determined via Vitek II, of which 28 (1%) were carbapenem resistant. Species identifications were determined via matrix-assisted laser desorption ionization time-of-flight mass spectrometry and carbapenemase gene detection via real-time PCR indicated that 23 (82.1%) isolates were Klebsiella pneumoniae (OXA-48-like, n = 7; NDM, n = 14; OXA-48-like and NDM, n = 2), four (14.2%) were Enterobacter cloacae complex (NDM, n = 3 and VIM, n = 1), and one (3.5%) was Escherichia coli (NDM). Sequencing of carbapenemase gene amplicons from a subset of isolates identified blaNDM-1, blaOXA-48, and blaVIM-4 alleles. Strain typing detected seven different K. pneumoniae variable number tandem repeat types, consistent with mostly sporadic occurrences along with limited clonal spread. E. cloacae complex isolates were diverse by pulsed-field gel electrophoresis, with a maximum relatedness of 70%. Plasmid restriction fragment length polymorphism (pRFLP) revealed four distinct profiles associated with NDM-encoding plasmids that were positive for replicons of the FII(K)/FIB or FIB incompatibility (Inc) groups via PCR-based replicon typing. OXA-48-encoding IncL/M plasmids differed by two pRFLP bands. The results show diverse CPE produce OXA-48 and NDM-1 enzymes in Jordan and that the carbapenemase genes are distributed on diverse plasmids in Jordanian hospitals, with some limited evidence for related clusters occurring, emphasizing the need for strict infection control measures. |
| Databáze: | OpenAIRE |
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