Evidence that cowpea aphid-borne mosaic and blackeye cowpea mosaic viruses are two different potyviruses

Autor:	H. W. Rossel, C. Huguenot, R. I. Hamilton, G. Thottappilly, M. T. Furneaux
Rok vydání:	1993
Předmět:	Microbiological Techniques Serotype Aphid Plants Medicinal Mosaic virus viruses Cowpea mosaic virus Potyvirus Antibodies Monoclonal food and beverages Enzyme-Linked Immunosorbent Assay Fabaceae Biology biology.organism_classification Virology Antibodies Virus Serology Mosaic Viruses Plant virus
Zdroj:	Journal of General Virology. 74:335-340
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-74-3-335
Popis:	The immunoreactivity of a panel of polyclonal anti-bodies and monoclonal antibodies (MAbs) raised against African isolates of potyviruses from cowpea and African yam bean was examined in ELISAs. A serological study including reference isolates followed by further characterization in differential hosts resulted in separation of the potyviruses into two distinct serogroups, one containing blackeye cowpea mosaic virus (BlCMV) and the other containing cowpea aphid-borne mosaic virus (CAMV). Using biotin-labelled MAbs, the BlCMV isolates were further subdivided into two serotypes and the CAMV isolates into five serotypes. Because both BlCMV and CAMV induce a very similar mosaic disease in cowpea, different ELISA procedures using mixed MAbs were evaluated and a single protocol was developed which allowed reliable diagnosis of both viruses.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a7020197c0fb6f69ae18c135df9d8a6 https://doi.org/10.1099/0022-1317-74-3-335 Zobrazit plný text záznamu