Leucine supplementation accelerates connective tissue repair of injured tibialis anterior muscle
| Autor: | Meiricris T. Silva, Eduardo de Oliveira Cruz Carlassara, Elen Haruka Miyabara, Paulo Alexandre Abrahamsohn, Marcelo G. Pereira, Dawit A. P. Gonçalves, Marcelo Saldanha Aoki, Anselmo Sigari Moriscot, Isis do Carmo Kettelhut |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Spasm Receptor Transforming Growth Factor-beta Type I Connective tissue lcsh:TX341-641 Hindlimb Smad2 Protein Biology Protein Serine-Threonine Kinases Smad2/3 Article transforming growth factor-β receptor type I Tibialis anterior muscle Myofibrils Leucine Internal medicine Myosin medicine Animals Regeneration Smad3 Protein Rats Wistar Muscle Skeletal connective tissue Nutrition and Dietetics Myosin Heavy Chains Tibia skeletal muscle injury Skeletal muscle SUPLEMENTAÇÃO ALIMENTAR Rats medicine.anatomical_structure Endocrinology Biochemistry Connective tissue metabolism repair leucine supplementation Dietary Supplements Collagen Myofibril lcsh:Nutrition. Foods and food supply Receptors Transforming Growth Factor beta Food Science |
| Zdroj: | Nutrients Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Volume 6 Issue 10 Pages 3981-4001 Nutrients, Vol 6, Iss 10, Pp 3981-4001 (2014) |
| ISSN: | 2072-6643 |
| Popis: | This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day) then, TA muscles from the left hind limb were cryolesioned and examined after 10 days. Although leucine supplementation induced increased protein synthesis, it was not sufficient to promote an increase in the cross-sectional area (CSA) of regenerating myofibers (p > 0.05) from TA muscles. However, leucine supplementation reduced the amount of collagen and the activation of phosphorylated transforming growth factor-β receptor type I (TβR-I) and Smad2/3 in regenerating muscles (p < 0.05). Leucine also reduced neonatal myosin heavy chain (MyHC-n) (p < 0.05), increased adult MyHC-II expression (p < 0.05) and prevented the decrease in maximum tetanic strength in regenerating TA muscles (p < 0.05). Our results suggest that leucine supplementation accelerates connective tissue repair and consequent function of regenerating TA through the attenuation of TβR-I and Smad2/3 activation. Therefore, future studies are warranted to investigate leucine supplementation as a nutritional strategy to prevent or attenuate muscle fibrosis in patients with several muscle diseases. |
| Databáze: | OpenAIRE |
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