Acetylcholine produces contractions mediated by the cyclooxygenase pathway in arterial vessels in the Chilean frog (Calyptocephalella gayi)
| Autor: | N. Urriola-Urriola, F. A. Moraga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Atropine
0301 basic medicine vascular reactivity Contraction (grammar) Indomethacin 030204 cardiovascular system & hematology 0302 clinical medicine acetilcolina lcsh:Botany lcsh:Zoology Muscarinic acetylcholine receptor lcsh:QL1-991 Chile lcsh:Science lcsh:QH301-705.5 biology reatividade vascular Arteries Anatomy lcsh:QK1-989 frog cyclooxygenase Anura medicine.symptom General Agricultural and Biological Sciences Acetylcholine medicine.drug medicine.medical_specialty ciclooxigenase atropine Muscarinic Antagonists atropina Cyclooxygenase pathway 03 medical and health sciences indomethacin Internal medicine medicine Animals Cyclooxygenase Inhibitors Muscarinic antagonist indometacina acetylcholine 030104 developmental biology Endocrinology lcsh:Biology (General) Prostaglandin-Endoperoxide Synthases Vasoconstriction biology.protein lcsh:Q Cyclooxygenase rã |
| Zdroj: | Brazilian Journal of Biology, Volume: 77, Issue: 4, Pages: 781-786, Published: 25 MAY 2017 Brazilian Journal of Biology v.77 n.4 2017 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE Brazilian Journal of Biology, Vol 77, Iss 4, Pp 781-786 (2017) |
| Popis: | Previous studies performed in marine fish (I. conceptionis and G. laevifrons) showed that indomethacin blocked arterial contraction mediated by acetylcholine (ACh). The objective of this study was to determine if contraction induced by acetylcholine is mediated by the cyclooxygenase pathway in several arterial vessels in the Chilean frog Calyptocephalella gayi. Arteries from the pulmonary (PA), dorsal (DA), mesenteric (MA) and iliac (IA) regions were dissected from 6 adult specimens, and isometric tension studies were done using dose response curves (DRC) for ACh (10-13 to 10-3 M) in presence of a muscarinic antagonist (Atropine 10-5 M) and an unspecific inhibitor of cyclooxygenases (Indomethacin, 10-5M). All the studied arteries exhibited vasoconstriction mediated by ACh. This vasoconstriction was abolished in the presence of atropine in DA, MA and IA and attenuated in PA. Indomethacin abolished the vasoconstriction in MA and attenuated the response in PA, DA and IA. Similar to marine fish, C. gayi have an ACh-mediated vasoconstrictor pattern regulated by muscarinic receptors that activate a cyclooxygenase contraction pathway. These results suggest that the maintenance in vasoconstrictor mechanisms mediated by ACh→COX →vasoconstriction is conserved from fish to frogs. Resumo Estudos feitos em peixes marinhos (I. conceptionis e G. laevifrons) têm demostrado que a indometacina bloqueia a contração arterial mediada por acetilcolina (ACh). O objetivo do presente estudo foi avaliar o efeito da via da ciclooxigenase na contração induzida por ACh em vasos arteriais da rã chilena Calyptocephalella gayi. Foram dissecadas regiões das artérias pulmonares (PA), dorsal (DA), mesentérica (MA) e ilíaca (IA) de seis espécimes adultos e realizados estudos de tensão isométrica utilizando curvas dose-resposta (CDR) de ACh (10-13 a 10-3 M) na presença de um antagonista muscarínico (atropina, 10-5 M) e um inibidor das ciclooxigenases (indometacina, 10-5 M). Todas as artérias evidenciaram uma resposta vasoconstritora mediada por ACh. Esta resposta vasoconstrictora foi suprimida na presença de atropina nas artérias DA, MA, IA e atenuada na PA. A indometacina suprimiu a vasoconstrição na artéria MA e atenuou a resposta nas artérias PA, DA e IA. Tal como os peixes marinhos, a C. gayi tem um padrão de vasoconstrição mediado por Ach que é regulado pelos receptores muscarínicos e pela ciclooxigenase. Estes resultados sugerem a conservação dos mecanismos vasoconstrictores mediados por ACh→COX em peixes e rãs. |
| Databáze: | OpenAIRE |
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