Use of Short Duplexes for the Analysis of the Sequence-Dependent Cleavage of DNA by a Chemical Nuclease, a Manganese Porphyrin
| Autor: | Sophie Mourgues, Geneviève Pratviel, Adam Kupan, Bernard Meunier |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Manganese
Guanine Porphyrins Base Sequence Chemistry Base pair DNA damage Oligonucleotide Stereochemistry Organic Chemistry Oligonucleotides DNA Photochemistry Biochemistry Porphyrin chemistry.chemical_compound Electron transfer Deoxyribose Molecular Medicine Oxidation-Reduction Molecular Biology DNA Damage |
| Zdroj: | ChemBioChem. 6:2326-2335 |
| ISSN: | 1439-4227 |
| DOI: | 10.1002/cbic.200500254 |
| Popis: | A manganese porphyrin, manganese(III)-bis(aqua)-meso-tetrakis(4-N-methylpyridiniumyl)porphyrin, in the presence of KHSO5 is able to perform deoxyribose or guanine oxidation depending on its mode of interaction with DNA. These two reactions involve an oxygen-atom transfer or an electron transfer, respectively. The oxidative reactivity of the manganese-oxo porphyrin was compared on short oligonucleotide duplexes of different sequences. The major mechanism of DNA damage is due to deoxyribose hydroxylation at a site of strong interaction, an (A.T)3 sequence. Guanine oxidation by electron transfer was found not to be competitive with this major mechanism. It was found that a single intrastrand guanine was three orders of magnitude less reactive than an (A.T)3 sequence. The reactivity of a 5'-GG sequence was found to be intermediate and was estimated to be two orders of magnitude less than that of an (A.T)3 site. Short oligonucleotide duplexes, as double-stranded-DNA models, proved to be convenient tools for the study of the comparative reactivity of this reagent toward different sequences of DNA. However, they showed a particular reactivity at their terminal base pairs (the "end effect") that biased their modeling capacity for double-helix-DNA models. |
| Databáze: | OpenAIRE |
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