Comparison of mesenchymal stromal cells from human bone marrow and adipose tissue for the treatment of spinal cord injury
| Autor: | Yinhai Chen, Shao-xiong Min, Hui Zhang, Bo Yu, Anmin Jin, Zhilai Zhou, Bing He |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Angiogenesis Cell Survival Immunology Adipose tissue Neovascularization Physiologic Bone Marrow Cells Mesenchymal Stem Cell Transplantation Rats Sprague-Dawley Neurotrophic factors Cell Movement medicine Immunology and Allergy Animals Humans Spinal cord injury Genetics (clinical) Spinal Cord Injuries Cell Proliferation Transplantation business.industry Brain-Derived Neurotrophic Factor Macrophages Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Ectodysplasins Middle Aged medicine.disease Spinal cord Rats medicine.anatomical_structure Oncology Adipose Tissue Spinal Cord Hepatocyte growth factor Female Bone marrow business medicine.drug |
| Zdroj: | Cytotherapy. 15(4) |
| ISSN: | 1477-2566 |
| Popis: | Background aims Bone marrow and subcutaneous adipose tissue are both considered prospective sources of mesenchymal stromal cells (MSCs), which can be used in cell therapy for spinal cord injury (SCI). The present study investigated whether human adipose tissue-derived mesenchymal stromal cells (hADSCs) transplanted into a rat model of SCI would lead to similar or improved neurologic effects compared with human bone marrow-derived mesenchymal stromal cells (hBMSCs). Methods hADSCs and hBMSCs were isolated from five adult donors. These MSCs were characterized using flow cytometry, immunocytochemistry, real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Immediately after SCI, 2 × 105 hBMSCs or hADSCs were injected into the injured spinal cord. Locomotor function, cell survival and differentiation, spinal cord tissue morphology and brain-derived neurotrophic factor (BDNF) expression were compared between groups. Results hADSCs and hBMSCs showed similar surface protein expression, and hADSCs showed higher proliferative activity with higher expression of vascular endothelial cell growth factor, hepatocyte growth factor and BDNF than hBMSCs. After transplant, both hADSCs and hBMSCs migrated within the injured spinal cord without differentiating into glial or neuronal elements. Administration of hADSCs was associated with marked changes in the SCI environment, with significant increases in BDNF levels. This was simultaneously associated with increased angiogenesis, preserved axons, decreased numbers of ED1-positive macrophages and reduced lesion cavity formation. These changes were accompanied by improved functional recovery. Conclusions The present results suggest that hADSCs would be more appropriate for transplant to treat SCI than hBMSCs. |
| Databáze: | OpenAIRE |
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