Cycling of intracellular pH during cell division of Xenopus embryos is a cytoplasmic activity depending on protein synthesis and phosphorylation
| Autor: | Michel Charbonneau, Nathalie Grandin |
|---|---|
| Přispěvatelé: | Laboratoire d'Innovation pour les Technologies des Energies Nouvelles et les nanomatériaux (LITEN), Institut National de L'Energie Solaire (INES), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie et Génétique du Développement, Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Grandin, Nathalie, Charbonneau, Michel |
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Male
Cytoplasm MESH: Hydrogen-Ion Concentration Embryo Nonmammalian Time Factors MESH: Diterpenes Cell division [SDV]Life Sciences [q-bio] Xenopus [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Biological Factors Xenopus laevis 0302 clinical medicine MESH: Ovum MESH: Animals Cycloheximide Phosphorylation MESH: Cycloheximide Genetics 0303 health sciences MESH: Kinetics Articles Hydrogen-Ion Concentration Cell biology [SDV] Life Sciences [q-bio] medicine.anatomical_structure MESH: Cell Division Female Diterpenes Cell Division MESH: Cell Nucleus MESH: Adenine Intracellular pH Maturation promoting factor Mitosis [SDV.CAN]Life Sciences [q-bio]/Cancer MESH: Aphidicolin [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Models Biological MESH: Oocytes 03 medical and health sciences Aphidicolin [SDV.CAN] Life Sciences [q-bio]/Cancer MESH: Xenopus laevis medicine [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Animals [SDV.BC] Life Sciences [q-bio]/Cellular Biology Ovum 030304 developmental biology Cell Nucleus Germinal vesicle MESH: Phosphorylation Adenine MESH: Cytoplasm MESH: Time Factors MESH: Models Biological MESH: Embryo Nonmammalian Oocyte activation [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology MESH: Mitosis biology.organism_classification Oocyte MESH: Male Kinetics Fertilization Oocytes biology.protein MESH: Biological Factors MESH: Fertilization MESH: Female 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 1990, pp.523-32 Journal of Cell Biology, 1990, pp.523-32 The Journal of Cell Biology |
| ISSN: | 0021-9525 1540-8140 |
| Popis: | International audience; In Xenopus embryos, the successive and rapid cell divisions that follow fertilization are accompanied by periodic oscillations of intracellular pH (pHi). Cycling of pHi occurs in phase with several other oscillatory activities, namely nuclear divisions, M phase-promoting factor (MPF) activity, and surface contraction waves (SCWs). We report that treatments that abolish cycling of MPF activity and the SCWs also suppress the pHi oscillations, whereas those that block cell division without affecting neither MPF activity nor the SCWs do not suppress the pHi oscillations. Experiments on enucleated oocytes, matured in vitro and activated, demonstrated that the activity governing the rhythmicity of the pHi oscillations resided in the cytoplasm of the oocyte. In this respect, the activity responsible for the pHi oscillations was different from that which drives the SCWs, which necessitated the presence of the oocyte germinal vesicle (Ohsumi et al., 1986), but more closely resembled MPF activity that did not require the presence of the oocyte germinal vesicle (Dabauvalle et al., 1988). In mature eggs enucleated at the time of egg activation, the pHi oscillations were similar to those in control nucleated eggs, whereas the period between two peaks of SCWs was 35-60 min vs. 20-35 min in nucleated control eggs. Previous studies had shown that the periodicity of SCWs was larger in anucleate egg fragments than in their nucleate counterparts (Sakai and Kubota, 1981), the difference being on the order of 6-15 min (Shinagawa, 1983). However, in these previous studies, enucleation was performed 30-50 min after fertilization. Our results clearly demonstrate that the periodicity of the SCWs is lengthened when the interval between egg activation and enucleation is shortened, thereby providing an easier way to assess the nuclear dependency of the SCWs. Finally, the various possibilities concerning the role of pHi cycling during cell division are discussed. |
| Databáze: | OpenAIRE |
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