Poly (rC) binding protein 2 interacts with VP0 and increases the replication of the foot-and-mouth disease virus
| Autor: | Shaozu Fu, Li Dan, Zhang Jing, Yanchun He, Yi Ru, Xiangtao Liu, Lulu Li, Haixue Zheng, Wenping Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Swine viruses animal diseases Immunology Apoptosis Plasma protein binding Virus Replication Article Virus Cell Line Mice Viral Proteins 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Animals lcsh:QH573-671 Gene knockdown biology lcsh:Cytology Binding protein RNA-Binding Proteins virus diseases Interferon-beta Cell Biology biochemical phenomena metabolism and nutrition biology.organism_classification Virology 030104 developmental biology Foot-and-Mouth Disease Virus Cell culture 030220 oncology & carcinogenesis Proteolysis Signal transduction Foot-and-mouth disease virus Protein Binding Signal Transduction |
| Zdroj: | Cell Death & Disease Cell Death and Disease, Vol 10, Iss 7, Pp 1-14 (2019) |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-019-1751-6 |
| Popis: | Foot-and-mouth disease virus (FMDV) causes a highly contagious and debilitating disease in cloven-hoofed animals, which leads to devastating economic consequences. Previous studies have reported that some FMDV proteins can interact with host proteins to affect FMDV replication. However, the influence of the interactions between FMDV VP0 protein and its partners on FMDV replication remains unknown. In this study, we found that the overexpression of poly (rC) binding protein 2 (PCBP2) promoted FMDV replication, whereas the knockdown of PCBP2 suppressed FMDV replication. Furthermore, PCBP2 can interact with FMDV VP0 protein to promote the degradation of VISA via the apoptotic pathway. Further studies demonstrated that FMDV VP0 protein enhanced the formation of the PCBP2-VISA complex. Ultimately, we found that the degradation of VISA was weaker in PCBP2-knockdown and FMDV VP0-overexpressing cells, or FMDV VP0-knockdown cells than in the control cells. Summarily, our data revealed that the interaction between PCBP2 and VP0 could promote FMDV replication via the apoptotic pathway. |
| Databáze: | OpenAIRE |
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