A PiggyBac mediated approach for lactoferricin gene transfer in bovine mammary epithelial stem cells for management of bovine mastitis
| Autor: | Dong Kee Jeong, Simrinder Singh Sodhi, Do Luong Huynh, Sung Jong Oh, Sung Woo Kim, Kafil Hussain, Mrinmoy Ghosh, Amit Kumar Singh, Nam Eun Kim, Sung-Jin Lee, Neelesh Sharma |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.disease_cause law.invention 03 medical and health sciences chemistry.chemical_compound antibacterial activity law Lactoferricin medicine Escherichia coli 030102 biochemistry & molecular biology Chemistry Electroporation PiggyBac Transfection Molecular biology 030104 developmental biology Oncology PiggyBac Transposon System antibacterial milk peptide Recombinant DNA Stem cell Antibacterial activity bovine lactoferricin Research Paper bovine mastitis |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | The antibacterial and anti-inflammatory properties of lactoferricin have been ascribed to its ability to sequester essential iron. The objective of the study was to clone bovine lactoferricin (LFcinB) gene into PiggyBac Transposon vector, expression study in the bovine mammary epithelial stem cells (bMESCs) and also to determine the antimicrobial property of recombinant LFcinB against bovine mastitis-causing organisms. The PiggyBac-LFcinB was transfected into bMESCs by electroporation and a three fold of LFcinB secretion was observed in the transfected bMESCs medium by ELISA assay. Furthermore, the assessment of antimicrobial activity against mastitis causing pathogens Staphylococcus aureus and Escherichia coli demonstrated convincing evidence to prove strong antibacterial activity of LFcinB with 14.0±1.0 mm and 18.0±1.5 mm zone of inhibition against both organisms, respectively. The present study provides the convincing evidence to suggest the potential of PiggyBac transposon system to transfer antibacterial peptide into bMESCs or cow mammary gland and also pave the way to use bovine mammary gland as the bioreactors. Simultaneously, it also suggest toward commercial utilization of LFcinB bioreactor system in pharmaceutical industry. |
| Databáze: | OpenAIRE |
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