MOESM1 of Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production

Autor: Moreau, Céline, Tapin-Lingua, Sandra, Grisel, Sacha, Gimbert, Isabelle, Gall, Sophie Le, Meyer, Valérie, Petit-Conil, Michel, Jean-Guy Berrin, Cathala, Bernard, Villares, Ana
Rok vydání: 2019
DOI: 10.6084/m9.figshare.8312894.v1
Popis: Additional file 1: Fig S1. Monitoring of the recombinant production of PaLPMO9E in a 1.3 L bioreactor. Fig S2 a Image of the enzymatic treatment of birchwood Kraft fibers in the Tornado multiple chamber reactor. The enzymatic treatment was performed in parallel in four chambers using 7 g of cellulose fibers in each of them. b Aspect of the PaLPMO9E-treated bleached birchwood Kraft fibers after filtration. Fig S3 a HPAEC chromatograms showing the elution profile of oxidized and non-oxidized oligosaccharides and b quantification by HPAEC analysis of the soluble sugars released by the action of PaLPMO9E on starting bleached birchwood Kraft fibers (KF), PaLPMO9E-treated bleached birchwood Kraft fibers (KF-LPMO), Ultra Turrax dispersed PaLPMO9E-treated fibers (NFC0) and PaLPMO9E-treated fibers submitted to mechanical shearing (NFC1–3). Fig S4 Normalized HPSEC-RI chromatograms of solubilized fibers from starting bleached birchwood Kraft fibers (KF), PaLPMO9E-treated bleached birchwood Kraft fibers (KF-LPMO), Ultra Turrax dispersed PaLPMO9E-treated fibers (NFC0) and PaLPMO9E-treated fibers submitted to mechanical shearing (NFC1–3). Table S1. Characteristics (chemical shift position of the peaks, δ, and full width at half height, FWHH) of signals generated from the deconvolution at the C-4 region of the solid-state 13C CP/MAS NMR spectra of starting bleached birchwood Kraft fibers (KF), PaLPMO9E-treated bleached birchwood Kraft fibers (KF-LPMO), Ultra Turrax dispersed PaLPMO9E-treated fibers (NFC0) and PaLPMO9E-treated fibers submitted to mechanical shearing (NFC1–3).
Databáze: OpenAIRE