Trypanosoma cruzi: identification of a galactose-binding protein that binds to cell surface of human erythrocytes and is involved in cell invasion by the parasite
| Autor: | Rubén P. Laguens, Iván Sergio Marcipar, A.J Marcipar, P. Cabeza Meckert, Ariel Mariano Silber, Cintia Roodveldt |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Peanut agglutinin
Monosaccharide Transport Proteins Trypanosoma cruzi Blotting Western Immunology Fluorescent Antibody Technique Chromatography Affinity Mice chemistry.chemical_compound Lectins parasitic diseases medicine Animals Humans Bovine serum albumin chemistry.chemical_classification Mice Inbred BALB C biology Immune Sera Calcium-Binding Proteins Erythrocyte Membrane Galactose Heart Helminth Proteins General Medicine biology.organism_classification Immunohistochemistry Molecular biology Red blood cell Infectious Diseases medicine.anatomical_structure chemistry Biochemistry Concanavalin A Periplasmic Binding Proteins biology.protein Electrophoresis Polyacrylamide Gel Parasitology Endothelium Vascular Rabbits PMSF Antibody Glycoprotein |
| Zdroj: | Experimental Parasitology. 100:217-225 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/s0014-4894(02)00013-9 |
| Popis: | Trypanosoma cruzi must invade mammalian host cells to replicate and complete its life cycle. Almost all nucleated mammalian cells can be invaded by the parasite following a receptor–ligand recognition as an early prerequisite. In this work, we describe a 67-kDa lectin-like glycoprotein that binds to desialylated human erythrocyte membranes in a galactose-dependent way. This protein is present on the parasite surface in both infective and non-infective stages of T. cruzi. More interestingly, we demonstrate by lectin-immuno-histochemistry assays that the 67 kDa protein is involved in the recognition of host-cell receptors in mouse cardiac tissue and human cardiac aortic endothelium and mammary artery tissue. Moreover, antibodies against the 67 kDa glycoprotein inhibit in vitro host-cell invasion by 63%. These data suggest that the 67 kDa glycoprotein in vivo is needed for host-cell invasion by T. cruzi. Index Descriptors and Abbreviations: Trypanosoma cruzi (T. cruzi), Red blood cell membranes, 67-kDa lectin-like glycoprotein (LLGP-67), Shed acute phase antigen (SAPA), Red blood cell ghosts (RBCG), Lythium diiodesalicylate (LIS), Total homogenate (TH), Phosphate buffered saline (PBS), Phosphate saline buffer, 0.01% Tween 20 (PBS-T), Phenylmethylsulphonyl fluoride (PMSF), Nα-p-Tosyl- l -lysinechloromethyl ketone (TLCK), Ultraviolet (UV), Concanavalin A (Con A), Peanut agglutinin (PNA), Tetramethylbenzidine (TMB), Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), Enzyme-linked immunosorbent assay (ELISA), Immunofluorescence asssay (IFA), Bovine serum albumin (BSA), Indirect hemagglutination assay (IHA), Dimethyl sulphoxide (DMSO). |
| Databáze: | OpenAIRE |
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